Epigallocatechin-3-gallate (EGCG) protects the oocytes from methyl parathion-induced cytoplasmic deformities by suppressing oxidative and endoplasmic reticulum stress,Pesticide Biochemistry and Physiology

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Epigallocatechin-3-gallate (EGCG) protects the oocytes from methyl parathion-induced cytoplasmic deformities by suppressing oxidative and endoplasmic reticulum stress
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.pestbp.2020.104588
Shweta Hegde ₁ , Keerthana Karunakar Poojary ₁ , Rhea Rasquinha ₁ , Daphne Norma Crasta ₁ , Divya Gopalan ₂ , Srinivas Mutalik ₂ , Sazada Siddiqui ₃ , Satish Kumar Adiga ₁ , Guruprasad Kalthur ₁

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Methyl parathion (MP) is a commonly used organophosphorus insecticide in commercial farming. It is well known that MP exposure can affect the function of nervous, respiratory, cardiovascular and reproductive systems. In our previous report we have demonstrated that MP exposure results in poor oocyte maturation and defective embryo development which is mainly mediated through oxidative stress. The present investigation was designed to explore whether using a potent free radical scavenger like Epigallocatechin-3-gallate (EGCG) can help in reducing the detrimental effects of MP on the oocytes. For the study, germinal vesicle (GV) stage oocytes collected from the ovaries of adult Swiss albino mice were subjected to in vitro maturation (IVM) in the presence or absence of MP (100 μg/mL) and/or EGCG (0.25 μM). MP significantly reduced the nuclear maturation rate, and resulted in poor cytoplasmic organization which was evident from the altered distribution pattern of mitochondria, endoplasmic reticulum and abnormal spindle organization. These changes were associated with significant elevation in oxidative stress and expression of ER stress markers such as 78 kDa Glucose regulated protein (GRP78) as well as X-box binding protein-1 (XBP1) in the oocytes. Further, the oocytes exposed to MP had lower activation rate and developmental potential. Supplementation of EGCG during IVM not only improved the nuclear maturation rate but also reduced the cytoplasmic abnormalities. These beneficial effects appear to be due to mitigation of oxidative and ER stress in oocytes. In conclusion, results of our study indicate that EGCG can help in alleviating MP-induced oocyte abnormalities.

中文翻译：

表没食子儿茶素-3-没食子酸酯 (EGCG) 通过抑制氧化和内质网应激保护卵母细胞免受甲基对硫磷诱导的细胞质畸形

甲基对硫磷 (MP) 是商业农业中常用的有机磷杀虫剂。众所周知，MP 暴露会影响神经、呼吸、心血管和生殖系统的功能。在我们之前的报告中，我们已经证明 MP 暴露导致卵母细胞成熟不良和胚胎发育缺陷，这主要是通过氧化应激介导的。本研究旨在探讨使用强效自由基清除剂如表没食子儿茶素-3-没食子酸酯 (EGCG) 是否有助于减少 MP 对卵母细胞的不利影响。在这项研究中，从成年瑞士白化小鼠的卵巢收集的生发囊泡 (GV) 期卵母细胞在存在或不存在 MP (100 μg/mL) 和/或 EGCG (0.25 μM) 的情况下进行体外成熟 (IVM) . MP显着降低细胞核成熟率，并导致细胞质组织不良，这从线粒体、内质网和异常纺锤体组织的分布模式改变中可见一斑。这些变化与氧化应激的显着升高和内质网应激标志物的表达有关，例如卵母细胞中的 78 kDa 葡萄糖调节蛋白 (GRP78) 以及 X-box 结合蛋白-1 (XBP1)。此外，暴露于 MP 的卵母细胞具有较低的活化率和发育潜力。在 IVM 期间补充 EGCG 不仅提高了核成熟率，而且减少了细胞质异常。这些有益作用似乎是由于减轻了卵母细胞中的氧化和内质网应激。综上所述，

更新日期：2020-07-01

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