The increasing Yezo sika deer (Cervus nippon yesoensis) population is creating a large problem. Yezo sika deer are an important blood meal source, and these deer contribute to the maintenance of tick populations. Theileria spp. infections in Yezo sika deer and T. orientalis infections in cows occur at high frequencies, and the same tick species infests both deer and cows. Therefore, a specific detection method to identify deer Theileria spp. is important. In this study, we establish a novel molecular detection method for identifying Theileria spp. from deer and tick samples using loop-mediated isothermal amplification (LAMP). This method targets a metalloprotease/cell division cycle protein gene homologue. Our LAMP protocol was able to detect deer Theileria and did not show cross reactivity with other closely related protozoan parasites, including T. orientalis. The LAMP method showed sensitivity and specificity equivalent to those of nested PCR performed on the same field samples from deer and ticks. These results demonstrate the applicability of LAMP to field surveys in which the detection of deer Theileria spp. is required. In conclusion, due to its simplicity, specificity, and reliability, we suggest our LAMP protocol as an appropriate method for routine surveys to detect Yezo sika deer and ticks infected with deer Theileria spp. parasites. Additionally, this LAMP method offers great promise as a useful tool to distinguish Yezo sika deer Theileria from related Theileria parasites present in livestock.

越来越Yezo梅花鹿（梅花鹿yesoensis）人口创造一个大问题。野三梅鹿是重要的血粉来源，这些鹿有助于维持of的种群。泰勒虫属 奶牛的野三叶草感染和东方三叶草感染以高频率发生，相同的tick虫感染鹿和牛。因此，一种特定的检测方法可鉴定出鹿Theileria spp。很重要 在这项研究中，我们建立了一种识别泰勒虫的新型分子检测方法spp。使用环介导的等温扩增（LAMP）从鹿和tick的样品中提取。该方法靶向金属蛋白酶/细胞分裂周期蛋白基因同源物。我们的LAMP方案能够检测到鹿泰勒虫，并且与其他紧密相关的原生动物寄生虫（包括东方锥虫）没有显示出交叉反应性。LAMP方法显示出的敏感性和特异性与对来自鹿和tick的相同田间样品进行的巢式PCR相当。这些结果证明了LAMP在野外调查中的适用性，在这种调查中，发现了鹿泰勒虫spp。是必须的。总而言之，由于其简单，特异性和可靠性，我们建议将LAMP方案作为常规调查的合适方法，以检测出被Yethe sika鹿和被Theerria spp感染的壁虱。寄生虫。此外，该灯的方法提供了很大的希望成为一个有用的工具来区分Yezo梅花鹿泰勒从与泰勒有寄生虫存在于家畜。