As a large group of natural product with significant biological activities, triterpenoid secretion is of particular importance towards its bioproduction. Due to the lack of specific transporters, most triterpenoids are naturally accumulated inside the cells. In this study, by taking an antitumor triterpenoid ganoderic acid 3-hydroxy-lanosta-8,24-dien-26 oic acid (GA-HLDOA) as example, we discovered that addition of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) or 2,6-dimethyl-β-cyclodextrin (DM-β-CD) enable the fast and sufficient secretion of GA-HLDOA by the recombinant Saccharomyces cerevisiae strain as constructed in our previous study. In addition, these cyclodextrins (CDs) could not enter into cells, while no significant change of the cell membrane fluidity was observed after CDs treatment. This discovery provides a potential generally applicable method for triterpenoid secretion.

作为一大类具有重要生物活性的天然产物，三萜类化合物的分泌对其生物生产特别重要。由于缺乏特定的转运蛋白，大多数三萜类化合物自然堆积在细胞内。本研究以抗肿瘤三萜灵酸3-羟基-羊毛脂8,24-二烯-26酸（GA-HLDOA）为例，发现添加了2-羟丙基-β-环糊精（HP-β -CD）或2,6-二甲基-β-环糊精（DM-β-CD）可通过重组酿酒酵母快速而充分地分泌GA-HLDOA如我们先前研究中构建的菌株。另外，这些环糊精（CD）不能进入细胞，而在CD处理后未观察到细胞膜流动性的显着变化。该发现为三萜类化合物的分泌提供了潜在的普遍适用的方法。