Cytokine mRNA and protein expression by cell cultures of epithelial ovarian cancer—Methodological considerations on the choice of analytical method for cytokine analyses,American Journal of Reproductive Immunology

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Cytokine mRNA and protein expression by cell cultures of epithelial ovarian cancer—Methodological considerations on the choice of analytical method for cytokine analyses
American Journal of Reproductive Immunology ( IF 2.5 ) Pub Date : 2020-04-19 , DOI: 10.1111/aji.13249
Pernilla Israelsson _{1,

2} , Eva Dehlin ₁ , Ivan Nagaev ₁ , Eva Lundin ₃ , Ulrika Ottander ₂ , Lucia Mincheva‐Nilsson ₁

Affiliation

To get a comprehensive picture of cytokine expression in health and disease is difficult, cytokines are transiently and locally expressed, and protein analyses are burdened by biological modifications, technical issues, and sensitivity to handling of samples. Thus, alternative methods, based on molecular techniques for cytokine mRNA analyses, are often used. We compared cytokine mRNA and protein expression to evaluate whether cytokine mRNA profiles can be used instead of protein analyses.

中文翻译：

上皮性卵巢癌细胞培养物中细胞因子mRNA和蛋白质表达—细胞因子分析方法选择的方法学考虑

要全面了解健康和疾病中细胞因子的表达情况是困难的，细胞因子是瞬时和局部表达的，蛋白质分析受到生物学修饰，技术问题以及对样品处理的敏感性的困扰。因此，经常使用基于分子技术进行细胞因子mRNA分析的替代方法。我们比较了细胞因子mRNA和蛋白质表达，以评估是否可以使用细胞因子mRNA配置文件代替蛋白质分析。

更新日期：2020-04-19

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