Bacterial isolates Pseudomonas aeruginosa BR, Alcaligenes faecalis SW and Escherichia coli EC from a microbial fuel cell (MFC) were cocultured with each other. The isolates were added in a specific sequence one after the other (two cultures in one reactor). The study helped us conceptualise the synergistic and antagonistic behaviour of bacterial isolates and also emphasised the sequence in which a culture can be introduced in an anode chamber. The coculture conditions were evaluated on the basis of redox activity, electron transfer rate, columbic efficiency, and internal resistances. A good syntropy in terms of power production was found between P. aeruginosa BR and E. coli EC that recorded a power generation of 207.593 ± 1.705 µW/m2. The addition of any culture in any sequence affected the performance of P. aeruginosa BR. This culture when added as a secondary culture in the reactor enhanced the performance of that reactor. The sequence of addition of a culture in a reactor affected the performance in the combinations of (a) P. aeruginosa BR and A. faecalis SW and (b) A. faecalis SW and E. coli EC.

中文翻译：

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微生物燃料电池中顺序添加的生物催化共培养物的生物电化学行为

来自微生物燃料电池 (MFC) 的细菌分离株铜绿假单胞菌 BR、粪产碱杆菌 SW 和大肠杆菌EC 相互共培养。分离物以特定的顺序一个接一个地加入（在一个反应​​器中进行两种培养）。该研究帮助我们将细菌分离物的协同和拮抗行为概念化，并强调了将培养物引入阳极室的顺序。根据氧化还原活性、电子转移率、库仑效率和内阻评估共培养条件。在 P. aeruginosa BR 和 E.coli EC 之间发现了发电方面的良好同步，记录发电量为 207.593 ± 1.705 µW/m2。以任何顺序添加任何培养物都会影响铜绿假单胞菌 BR 的性能。这种培养物当作为反应器中的二次培养物添加时提高了该反应器的性能。在反应器中添加培养物的顺序影响 (a) 铜绿假单胞菌 BR 和 A. faecalis SW 和 (b) A. faecalis SW 和大肠杆菌 EC 的组合的性能。