People have affection for confectionary products. Confectionery products are of two types, baker and sugar confectionary. Dark chocolates belong to sugar confectionery class. The present invention was carried out on the preparation of synbiotic dark chocolates. Synbiotics are food products that contain both prebiotics and probiotic microorganisms, wherein prebiotics encourage the growth of probiotics. The synbiotic dark chocolates were amended with flax seeds (Linum usitatissimum L.) as a prebiotic for LAB. Flax seed contains fiber and phenolic antioxidants which makes them prebiotic source. The isolated LAB culture, which was identified as Leuconostoc mesenteroides based on morphological, biochemical tests and MALDI-TOF, showed the properties of a probiotic culture viz., tolerance to sodium chloride, bile salt, pH, and temperature, sensitivity to antibiotics, nonhemolytic and production of hydrogen peroxide. Cytotoxic activity of the cell free supernatant was assessed against MDA MB 231 and neuroblastoma cell line. Probiotic strain showed 48% and 30% cytotoxicity against MDA MB 231 and neuroblastoma cell line. The synbiotic chocolate was found to have more antioxidant activity, i.e. 90 U/mL by DPPH assay and 200 (μg Trolox/mL) by FRAP assay. The synbiotic chocolate prepared will be beneficial for the gut health of the humans and will also have excellent nutritional value.

中文翻译：

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以亚麻籽（Linum usitatissimum L.）强化的益生糖糖果。

人们喜欢糖果产品。糖果产品有两种类型，面包和糖食。黑巧克力属于糖食类别。本发明是针对共生黑巧克力的制备而进行的。合生元是既包含益生元又包含益生菌微生物的食品，其中益生元促进益生菌的生长。将共生黑巧克力与亚麻籽（亚麻（Linum usitatissimum L.））进行了修改，作为LAB的益生元。亚麻籽含有纤维和酚类抗氧化剂，使它们成为益生元。分离的LAB培养物，被鉴定为肠膜亮绿隐球菌基于形态学，生化测试和MALDI-TOF，显示了益生菌培养物的特性，即对氯化钠，胆汁盐，pH和温度的耐受性，对抗生素的敏感性，非溶血性和过氧化氢的产生。评估了无细胞上清液对MDA MB 231和成神经细胞瘤细胞系的细胞毒活性。益生菌菌株对MDA MB 231和神经母细胞瘤细胞系显示48％和30％的细胞毒性。发现该合生巧克力具有更大的抗氧化活性，即通过DPPH测定为90 U / mL，通过FRAP测定为200（μgTrolox / mL）。制备的共生巧克力将有益于人类肠道健康，并具有出色的营养价值。