During the last 10 to 15 yr, in vitro research to predict antral follicle growth and oocyte maturation has delivered interesting advances in the knowledge of processes regulating follicle growth and developmental competence of oocytes. This review discusses the contribution of cumulus and mural granulosa cells in the process of oocyte maturation and cumulus expansion in cumulus–oocyte complexes (COCs) from follicles of different sizes and shows that differences in gene expression in oocytes, granulosa, and theca cells of small and large follicles impact the success of in vitro blastocyst development. In addition, the molecular mechanisms by which COC metabolism and antioxidant defense provide oocyte competence are highlighted. Furthermore, new insights and perspectives on molecular and cellular regulation of in vitro oocyte maturation are emphasized.

在过去的 10 到 15 年中，预测窦卵泡生长和卵母细胞成熟的体外研究在调节卵母细胞生长和发育能力的过程的知识方面取得了有趣的进展。本综述讨论了卵丘和壁颗粒细胞在来自不同大小卵泡的卵丘 - 卵母细胞复合体 (COC) 中卵母细胞成熟和卵丘扩张过程中的贡献，并表明小卵母细胞、颗粒细胞和卵泡膜细胞中基因表达的差异。和大卵泡影响体外囊胚发育的成功。此外，还强调了 COC 代谢和抗氧化防御提供卵母细胞能力的分子机制。此外，