Antioxidants are the radical scavengers that inhibit peroxidation and other free-radical processes, which in return safeguard different organisms from various diseases attributed to radical reactions. Synthetic antioxidants inhibit free radicals, but they also have harmful side effects. However, mycochemicals of natural fungal origin are safe and best substitutes for harmful synthetic chemical antioxidants. The prime objectives of the study include appropriate qualitative and quantitative mycochemical screening, antioxidant potential, and chemical composition of Snef1216 (Penicillium chrysogenum). The study has used aluminium chloride colourimetric method, Folin–Ciocalteu reagent assay, and DPPH (1,1-diphenyl-1-picrylhydrazyl) for analysis of total flavonoid content and phenol content and antioxidant activity, respectively. However, the presence of biologically active compounds was screened through gas chromatography-mass spectrometry (GC-MS). Quantitative analysis demonstrated the existence of flavonoids, glycosides, flavones, saponins, phenols, and catecholic tannins excluding alkaloids, terpenoids, steroids, and gallic tannins. The outcomes exposed total flavonoid content and phenolic content in P. chrysogenum were 85.31 ± 1.23 mg·QE/g and 135.77 ± 1.14 mg·GAE/g, respectively. Snef1216 (P. chrysogenum) displayed the highest free-radical scavenging activity with 63.86% inhibition of DPPH. The analysis confirms that Snef1216 (P. chrysogenum) is an alternative source of natural antioxidants. The obtained data have provided the foundation for its use in agricultural, environmental, and pharmaceutical industries.

中文翻译：

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发酵菌株Snef1216（产黄青霉）的真菌化学筛选和分析，抗氧化活性和生化组成。

抗氧化剂是抑制过氧化和其他自由基过程的自由基清除剂，反过来又保护不同的生物免受自由基反应引起的各种疾病的侵害。合成抗氧化剂可抑制自由基，但它们也具有有害的副作用。但是，天然真菌来源的霉菌化学品是安全的，并且是有害合成化学抗氧化剂的最佳替代品。该研究的主要目标包括适当的定性和定量真菌化学筛选，抗氧化潜力以及Snef1216（产黄青霉（Penicillium chrysogenum））。该研究使用了氯化铝比色法，Folin–Ciocalteu试剂测定法和DPPH（1,1-二苯基-1-picylhydrazyl）分别分析了总黄酮含量，苯酚含量和抗氧化活性。但是，通过气相色谱-质谱（GC-MS）筛选了生物活性化合物的存在。定量分析表明，除了生物碱，萜类化合物，类固醇和没食子鞣质外，还存在类黄酮，糖苷，黄酮，皂角苷，酚和儿茶酚单宁。产黄青霉的总黄酮含量和酚含量分别为85.31±1.23 mg·QE / g和135.77±1.14 mg·GAE / g。Snef1216（黄。）表现出最高的自由基清除活性，对DPPH的抑制率为63.86％。分析证实，Snef1216（P. chrysogenum）是天然抗氧化剂的替代来源。所获得的数据为其在农业，环境和制药行业中的使用提供了基础。