Biocides are frequently used in the manufacturing of textiles that are in direct contact with human skin. Recently regulated biocides do not have validated methods for testing; so, their presence cannot be estimated in the consumer products. Hence a rapid method was developed for the separation and quantitative analysis of biocide contents (2-methyl-4-isothaizolin (MIT), 5-chloro-2-methyl-4-isothaizolin-3-one (CIT), 2-octo-4-isothaizolin-3-one (OIT), and 5-chloro-2-(2,4-dichlorophenxy) phenol (triclosan)) from the textile test specimens. Test specimens were extracted with methanolic sonication and purified by centrifugation and filtration. Biocide contents were separated at C18 column with 0.4% acetic acid: methanol (1 : 1 v/v) under isocratic mode and detected at 280 nm wavelength. Pretreatment factors such as extraction solvent, extraction method, dilution ratio, and extraction time were optimized initially and plotted calibration curve showed regression (r² ≥ 0.9995) in the range of 1.0–5.0 mg L⁻¹. Recoveries were between 95% and 108% with the relative standard deviation ≤ 4%. Limits of detection (LODs) were between 0.06 mg L⁻¹ and 0.12 mg L⁻¹ and limits of quantification (LOQs) were between 0.21 mg L⁻¹ and 0.38 mg L⁻¹. From the results, conclusion was made that the method can achieve the purpose of quantitative detection and the analysis of real test specimens verified the reliability of this method.

中文翻译：

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开发一种从LC-DAD上的纺织品测试样品中提取和分析杀菌剂含量的高效分析方法。

杀生物剂常用于制造与人类皮肤直接接触的纺织品。最近管制的杀生物剂没有经过验证的测试方法；因此，无法估计它们在消费产品中的存在。因此，开发了一种快速的方法来分离和定量分析杀菌剂的含量（2-甲基-4-异噻唑啉（MIT），5-氯-2-甲基-4-异噻唑啉-3-one（CIT），2-辛基-纺织品测试样品中的4-isothaizolin-3-one（OIT）和5-chloro-2-（2,4-dichlorophenxy）phenol（triclosan）。用甲醇超声提取试样，并通过离心和过滤纯化。在等度模式下，在C18柱上用0.4％的乙酸：甲醇（1：1 v / v）分离杀生物剂的含量，并在280 nm波长处检测。预处理因素，例如萃取溶剂，[R ²  ≥0.9995）在1.0-5.0毫克L的范围^-1。回收率在95％到108％之间，相对标准偏差≤4％。检测限（LOD）在0.06 mg L ^-1至0.12 mg L ^-1之间，定量限（LOQ）在0.21 mg L ^-1至0.38 mg L ^-1之间。结果表明，该方法可以达到定量检测的目的，对真实试样的分析证明了该方法的可靠性。