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Comparison of the Antioxidant Activities and Phenolic Content of Five Lonicera Flowers by HPLC-DAD/MS-DPPH and Chemometrics.
International Journal of Analytical Chemistry ( IF 1.5 ) Pub Date : 2020-04-01 , DOI: 10.1155/2020/2348903
Rong-Rong Zhou 1 , Xue-Hui Liu 2 , Lin Chen 2 , Jian-Hua Huang 2 , Xue-Juan Liang 2 , Dan Wan 2 , Shui-Han Zhang 2 , Lu-Qi Huang 1, 3
Affiliation  

The Lonicera plants (family Caprifoliaceae) with strong antioxidant activity are used as potential health-supporting phytochemicals. Studying the detailed relationships between bioactive compounds and their antioxidant activity is important for further comprehensive development and application of them. In this paper, the antioxidant capacities and compositions of five species of Lonicera flowers were investigated by using the online HPLC-DAD/MS-DPPH method. Results indicated that the samples contained higher amounts of phenols had better antioxidant activity. Furthermore, principal component analysis and linear regression were further used to analyze the correlations between antioxidant capacity and compounds and find the compounds having higher contribution to antioxidant activity. 5-O-Caffeoylquinic acid, 4-O-caffeoylshikimic acid, methyl-5-O-caffeoylquinate, 1,4-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid were screened as stronger antioxidant candidates. In this study, HPLC-DAD/MS and antioxidant activity methods were combined together to analyze the compounds’ information and activity assays of Lonicera, which might provide more evidence for its quality control.

中文翻译:

HPLC-DAD / MS-DPPH和化学计量学比较五种忍冬花的抗氧化活性和酚类含量。

具有强抗氧化活性的忍冬属植物(忍冬科)被用作潜在的对健康有益的植物化学物质。研究生物活性化合物与其抗氧化活性之间的详细关系对于进一步全面开发和应用它们具有重要意义。本文研究了五种忍冬植物的抗氧化能力和组成通过在线HPLC-DAD / MS-DPPH方法研究了花朵。结果表明,所含酚含量较高的样品具有较好的抗氧化活性。此外,进一步使用主成分分析和线性回归分析抗氧化剂能力与化合物之间的相关性,发现对抗氧化剂活性具有更高贡献的化合物。筛选了5-O-咖啡酰基奎宁酸,4-O-咖啡酰基shi草酸,-5-O-咖啡酰基奎宁酸甲酯,1,4-二-O-咖啡酰基奎宁酸和3,4,5-三-O-咖啡酰基奎宁酸抗氧化剂的候选人。本研究将HPLC-DAD / MS和抗氧化活性方法结合起来分析忍冬属植物的化合物信息和活性测定,这可能为其质量控制提供更多证据。
更新日期:2020-04-01
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