Field trials in six agricultural sites were carried out to investigate the dissipation and residue levels of pyridaben in kiwifruit. Each sample was extracted with acetonitrile, purified with octadecylsilane and analyzed with high-performance liquid chromatography-tandem mass spectrometry. The method had good linearity (R² > 0.99), accuracy (recoveries of 78.53–98.00%) and precision (relative standard deviation of 0.86–6.11%). The dissipation of pyrdaben in kiwifruit followed first-order kinetics with a half-life < 8 d, and terminal residues in kiwifruit were lower than 0.5 mg/kg after 14 d of application. Risk assessment indicated that both chronic and acute dietary intake risk values were far below 100%, suggesting that pyridaben residues in kiwifruit were relatively safe to humans. Moreover, the effects of traditional household processes on kiwifruit were investigated. The processing factors (PFs) indicated that peeling and peeling-juicing processes could remove pyridaben residues from kiwifruit, and the former was more effective than the latter (PF at 0.15 vs. 0.51). Nevertheless, drying kiwifruit with an oven increased the amount of pyridaben (PF at 1.05). These results could provide guidance for the safe and reasonable use of pyridaben in agriculture and may be helpful for the Chinese government to determine maximum residue limit of pyridaben in kiwifruit.