Oils in food waste can pollute the environment and negatively affect human health. Biodegradation is a promising method for disposing of waste edible oils. In this study, an oil-degrading bacterium was isolated from kitchen waste for efficient degradation of edible oils. Its growth and oil degradation characteristics were investigated in basic salt medium with edible oils as the sole carbon and energy source; the triacylglycerol lipase gene (EC 3.1.1.3) was cloned and expressed in Escherichia coli. A novel oil-degrading bacterium assigned as IUMR B67 was successfully isolated. Morphological and molecular analyses revealed that strain IUMR B67 belongs to Kosakonia cowanii. After 144 h of incubation, the oil degradation rate at 37°C was 95.80%. Optimal conditions for IUMR B67 were recorded at 37°C and 0.1% NaCl with 0.1% ammonium sulfate supplementation. The lipase gene of strain IUMR B67 was determined to be 912 base pairs, and the lipase activity of the expressed protein was 3.02 U/mL, which was significantly higher than the control (P < 0.05). Overall, Kosakonia cowanii IUMR B67 is a novel edible oil-degrading strain that can hydrolyze oil via its lipase activity, which may be useful in the disposal of oils and oily food waste.

中文翻译：

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新型油降解菌株考萨尼亚牛（Kosakonia cowanii）IUMR B67的分离和表征以及降解酶的表达。

食物垃圾中的油脂会污染环境并对人类健康产生负面影响。生物降解是处理废弃食用油的一种有前途的方法。在这项研究中，从厨余中分离出一种降解油脂的细菌，以有效降解食用油。在以食用油为唯一碳源和能源的碱性盐培养基中研究了其生长和降解特性。克隆了三酰基甘油脂酶基因（EC 3.1.1.3）并在大肠杆菌中表达。成功分离出一种新型的石油降解细菌，命名为IUMR B67。形态和分子分析表明，菌株IUMR B67属于科萨尼亚牛。温育144小时后，在37℃下的油降解率为95.80％。IUMR B67的最佳条件记录在37°C和0.1％NaCl和0的条件下。1％硫酸铵补充。确定菌株IUMR B67的脂肪酶基因为912个碱基对，表达蛋白的脂肪酶活性为3.02 U / mL，明显高于对照（P <0.05）。总体而言，考萨尼亚牛cow IUMR B67是一种新型的可降解食用油菌株，可通过其脂肪酶活性水解油，可用于处理油和含油食物垃圾。