Aim and Objective: Lupus nephritis (LN) is one of the major complications of systemic lupus erythematosus (SLE). The specific mechanisms of pathogenesis, aggravation, and remission processes in LN have not been clarified but is of great need in the clinic. Using isobaric tags for relative and absolute quantitation (iTRAQ) technology to screen the functional proteins of LN in mice. Especially under intervention factors of lipopolysaccharide (LPS) and dexamethasone.

Methods: Mrl-lps mice were intervened with LPS, dexamethasone, and normal saline (NS) using intraperitoneal injection, and c57 mice intervened with NS as control. The anti-ANA antibody enzyme-linked immunosorbent assay (ELISA) was used to verify disease severity. Kidney tissue is collected and processed for iTRAQ to screen out functional proteins closely related to the onset and development of LN. Western blot method and rt-PCR (real-time Polymerase Chain Reaction) were used for verification.

Results: We identified 136 proteins that marked quantitative information. Among them, Hp, Igkv8-27, Itgb2, Got2, and Pcx proteins showed significant abnormal manifestations.

Conclusion: Using iTRAQ methods, the functional proteins Hp, Igkv8-27, Itgb2, Got2, and Pcx were screened out for a close relationship with the pathogenesis and development of LN, which is worth further study.

目的和目的：狼疮性肾炎（LN）是系统性红斑狼疮（SLE）的主要并发症之一。LN的发病机制，加重和缓解过程的具体机制尚未阐明，但在临床上非常需要。使用等压标记进行相对和绝对定量（iTRAQ）技术筛选小鼠LN的功能蛋白。特别是在脂多糖（LPS）和地塞米松的干预作用下。

方法：通过腹膜内注射对Mrl-lps小鼠进行LPS，地塞米松和生理盐水（NS）干预，对c57小鼠进行NS干预。抗ANA抗体酶联免疫吸附测定（ELISA）用于验证疾病的严重程度。收集肾脏组织并进行iTRAQ处理，以筛选出与LN的发生和发展密切相关的功能蛋白。使用蛋白质印迹法和rt-PCR（实时聚合酶链反应）进行验证。

结果：我们鉴定了136种标记定量信息的蛋白质。其中，Hp，Igkv8-27，Itgb2，Got2和Pcx蛋白显示出明显的异常表现。

结论：采用iTRAQ方法筛选出功能蛋白Hp，Igkv8-27，Itgb2，Got2和Pcx与LN的发生发展密切相关，值得进一步研究。