In this research communication we describe an innovative protocol that combines three pairs of primers, two from the literature and one designed in our laboratory, for application in triplex-PCR on somatic cell DNA to enable identification of the species origin (cow, sheep, goat) of cheeses and yogurts with a detection limit of 0.1%. Mislabeling was detected in 15 out of 40 cheeses and in 18 out of 40 yogurts tested. The suggested procedure is a quick and reliable tool for identifying the animal origin of cheeses and yogurts and it can be used to certify product reliability on the domestic and international market. Additionally, in combination with a serological test it can offer a reliable tool for detecting the presence of cow's whey.

中文翻译：

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应用三重 PCR 与 3 对引物的创新组合检测奶酪和酸奶中的牛奶动物来源

在本研究交流中，我们描述了一种创新方案，它结合了三对引物，两对来自文献，另一对在我们的实验室设计，用于体细胞 DNA 的三重 PCR 以识别物种来源（牛、绵羊、山羊) 的奶酪和酸奶的检测限为 0.1%。在 40 种奶酪中的 15 种和 40 种测试酸奶中的 18 种中检测到错误标签。建议的程序是识别奶酪和酸奶动物来源的快速可靠的工具，可用于证明产品在国内和国际市场上的可靠性。此外，结合血清学测试，它可以提供可靠的工具来检测牛乳清的存在。