Abstract

This study aims to identify the relative Copy number variation (CNV) associated with the litter size of Dazu black goats based on the unpublished CNV analytical results of our previously published sequencing data, in which the litter-size groups were classified into extreme low- and high-yield groups. Firstly, to compare the existence of valuable CNV in Dazu black goats with different fertility levels with mixed pools. We obtained 4992 and 4888 CNVs from the HY and LY, which overlapping 1461 genes, and classified on the original CNV type. Three genes [LOC108633278, PPP1R12A, and YIPF4] were observed in the intersection between the HY deletion and the LY duplication groups. Secondly, on individuals level, we identified a novel candidate CNV (Chr1_50215501, F_ST = 0.148, V_ST = 0.347) from 214 autosomal credible CNVs to be significant with litter size in the Dazu black goat, which located in the CBLB gene. This finding indicates the CBLB gene may affect the litter size of the Dazu black goats through structural variations, and Chr1_50215501 can be an effective genetic marker for marker-assisted selection breeding, and this study was also helps understand the molecular mechanism related to the goat litter size.

摘要

本研究旨在根据我们之前发表的测序数据的未发表的 CNV 分析结果，确定与大足黑山羊产仔数相关的相对拷贝数变异 (CNV)，其中产仔数组被分为极低和高收益群体。首先，比较不同生育水平的大足黑山羊混合池中有价值的CNV的存在情况。我们从 HY 和 LY 中获得了 4992 和 4888 个 CNV，它们重叠了 1461 个基因，并按原始 CNV 类型进行了分类。在 HY 缺失和 LY 重复组之间的交叉点中观察到三个基因 [ LOC108633278、PPP1R12A和YIPF4 ]。其次，在个体层面，我们确定了一个新的候选 CNV（Chr1_50215501，F _ST = 0.148, V _ST = 0.347) 来自 214 个常染色体可信 CNV 对位于CBLB基因的大足黑山羊的产仔数具有显着意义。这一发现表明CBLB基因可能通过结构变异影响大足黑山羊产仔数，而Chr1_50215501可以作为标记辅助选择育种的有效遗传标记，本研究也有助于了解与山羊产仔数相关的分子机制。尺寸。