Abstract

This study aimed to assess the effect of the insulin-like grow factor 1 (IGF-1) treatment during in vitro maturation on the gene expression and developmental ability of ovine oocytes. Ovine cumulus-oocyte complexes (COC) were matured in vitro without (control) or with the supplementation of IGF-1 (100 ng/ml) and then subjected to in vitro fertilization and culture. The rate of oocyte maturation and embryo development was recorded and expression of the selected genes (involved in the PI3K/Akt and apoptosis signaling) was assessed in the matured oocytes. The IGF-1 treatment significantly (p < .05) improved the oocyte maturation rate (%) as compared to the control (81.5 ± 2.40 vs. 73.6 ± 0.94). Similarly, as compared to the control, the IGF-1 treatment significantly (p < .05) improved the rate (%) of cleavage (54.7 ± 1.58 vs. 67.2 ± 3.65) and the formation of 4–8 cell embryos (30.7 ± 2.89 vs. 44.1 ± 4.01) and morula (20.7 ± 2.08 vs. 32.8 ± 2.78). The IGF-1 treatment significantly (p < .05) upregulated the expression of IGF1R, PI3KR1, AKT1 and BCL2 and downregulated the expression of GSK3β, FOXO3 and CASP9 in the matured oocytes. In conclusion, the IGF-1 treatment significantly improved the developmental competence of ovine oocytes through the regulation of the PI3K/Akt and apoptosis signaling.

摘要

本研究旨在评估体外成熟过程中胰岛素样生长因子 1 (IGF-1) 处理对绵羊卵母细胞基因表达和发育能力的影响。绵羊卵丘-卵母细胞复合物 (COC)在没有（对照）或补充 IGF-1（100 ng/ml）的情况下在体外成熟，然后进行体外受精和培养。记录卵母细胞成熟和胚胎发育的速率，并评估成熟卵母细胞中选定基因（参与 PI3K/Akt 和细胞凋亡信号传导）的表达。IGF-1 处理显着 ( p < .05) 与对照相比提高了卵母细胞成熟率 (%) (81.5 ± 2.40 vs. 73.6 ± 0.94)。同样，与对照相比，IGF-1 处理显着 ( p  < .05) 提高了卵裂率 (%) (54.7 ± 1.58 vs. 67.2 ± 3.65) 和 4-8 个细胞胚胎的形成 (30.7 ± 2.89 与 44.1 ± 4.01) 和桑椹 (20.7 ± 2.08 与 32.8 ± 2.78)。的IGF-1处理显著（p  <0.05）上调的表达IGF1R，PI3KR1，AKT1和BCL2和下调的表达GSK3β，FOXO3和CASP9在成熟的卵母细胞中。总之，IGF-1 处理通过调节 PI3K/Akt 和细胞凋亡信号显着提高了绵羊卵母细胞的发育能力。