Abstract Plants of the Plantago genus are widely used in Turkish folk medicine especially for the treatment of wound, abscess, and inflammation. The aqueous extract and five phenylethanoid glycosides acteoside (1), arenarioside (2), echinacoside (3), isoacteoside (4), and leucosceptoside A (5) isolated from the aerial parts and roots of Plantago holosteum Scop. (Plantaginaceae) were tested for their possible inhibitory activity against hyaluronidase, elastase, and collagenase, related to wound pathogenesis. Even though the aqueous extract prepared from the aerial parts (36.26%) and roots (47.01%) and the isolated compounds acteoside (29.13%), echinacoside (28.73%), and isoacteoside (31.69%) exerted a notable inhibition, arenarioside and leucosceptoside A were found inactive in the hyaluronidase enzyme inhibition test. Similar results were obtained from the collagenase enzyme inhibition test. The aqueous extract prepared from the aerial parts (31.09%) and roots (35.17%), echinacoside (25.13%), and isoacteoside (23.85%) exerted a notable inhibition in this test. However, none of the extracts and isolated compounds displayed elastase enzyme inhibitory activity. The experimental data demonstrated that P. holosteum displayed a remarkable enzyme inhibitory activity against hyaluronidase and collagenase. This paper is the first report regarding the in vitro enzyme inhibitory activity of P. holosteum.

中文翻译：

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从车前草中分离出的成分的酶抑制评估。

摘要 车前子属植物广泛用于土耳其民间医学，特别是用于治疗伤口、脓肿和炎症。从车前子的地上部分和根中分离出的水提取物和五种苯乙醇苷 (1)、槟榔苷 (2)、松果菊苷 (3)、异乙酸苷 (4) 和 leucosceptoside A (5)。（车前草科）对透明质酸酶、弹性蛋白酶和胶原酶的可能抑制活性进行了测试，这些活性与伤口发病机制有关。尽管从地上部分 (36.26%) 和根 (47.01%) 制备的水提物以及分离的化合物松果甙 (29.13%)、松果菊甙 (28.73%) 和异松果甙 (31.69%) 发挥了显着的抑制作用，但槟榔甙和白菊甙A 在透明质酸酶抑制试验中被发现无活性。从胶原酶抑制试验中获得了类似的结果。从地上部分（31.09%）和根（35.17%）、松果菊苷（25.13%）和异醋栗苷（23.85%）制备的水提物在该试验中发挥了显着的抑制作用。然而，没有一种提取物和分离的化合物显示出弹性蛋白酶抑制活性。实验数据表明，P. holosteum 对透明质酸酶和胶原酶具有显着的酶抑制活性。这篇论文是关于P. holosteum 体外酶抑制活性的首次报道。提取物和分离的化合物均未显示弹性蛋白酶抑制活性。实验数据表明，P. holosteum 对透明质酸酶和胶原酶具有显着的酶抑制活性。这篇论文是关于P. holosteum 体外酶抑制活性的首次报道。提取物和分离的化合物均未显示弹性蛋白酶抑制活性。实验数据表明，P. holosteum 对透明质酸酶和胶原酶具有显着的酶抑制活性。这篇论文是关于P. holosteum 体外酶抑制活性的首次报道。