Jatropha dioica is a popular plant used in Mexican herbal medicine to treat several diseases. Cytotoxicity, antimicrobial and antiviral activities have been reported for root extracts, while riolozatrione, 6-epi-riolozatrione, citlalitrione and jatrophatrione, among others, have been identified as the principal components. In this work, an HPLC/DAD method for the analysis of riolozatrione and other major compounds in extracts of different polarities was validated. The analysis was carried out on an AccQ-Tag column with a water-acetonitrile mixture as mobile phase. Flow rate was 0.2 mL/min, and the separation was carried out in gradient mode with UV detection set at 254 nm. The resulting method showed good reproducibility in both retention times and peak areas of riolozatrione, 6-epi-riolozatrione, citlalitrione and jatrophatrione, with relative standard variations lower than 4.5 and 10.5% respectively. In addition, this method provides a good performance for riolozatrione quantitation, with recoveries between 102 and 108% and RSDs lower than 2.5%. The polarity of the extracting solvent did not affect the performance of the chromatographic method. The developed method was applied for the analysis and quantification of riolozatrione in extracts of Jatropha dioica collected in several seasonal stages and years (2014-2017).

中文翻译：

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麻疯树提取物分析的HPLC方法验证。

麻疯树是墨西哥植物药中用于治疗多种疾病的流行植物。据报道根提取物具有细胞毒性，抗微生物和抗病毒活性，而赖奥氮杂酮，6-表-赖奥氮杂酮，瓜杂三酮和麻风树酮等已被确定为主要成分。在这项工作中，验证了HPLC / DAD方法用于分析不同极性提取物中的氟氮杂三酮和其他主要化合物。分析是在AccQ-Tag色谱柱上进行的，其中水-乙腈混合物为流动相。流速为0.2mL / min，并且分离以梯度模式进行，UV检测设置为254nm。所得方法在riolozatrione，6-epi-riolozatrione，citlalitrione和jatrophatrione的保留时间和峰面积上均显示出良好的重现性，相对标准偏差分别低于4.5和10.5％。此外，该方法为氟氮杂三酮定量提供了良好的性能，回收率在102％至108％之间，RSD低于2.5％。萃取溶剂的极性不影响色谱方法的性能。所开发的方法用于在几个季节和年份（2014-2017）收集的麻风树提取物中的利奥氮杂三酮的分析和定量。