This study aimed to detect genetically modified maize (GMM) in seeds of eleven imported maize hybrids grown in Jordan. We used promoter 35 S and T-nos terminator for general screening of transgenic materials. Conventional PCR detected the specific events for the screening of Bt 11, MON810, and Bt176 events. Seeds of eleven maize hybrids samples showed a positive response to the 35 S promoter; nine out of eleven showed a positive response for T-nos terminator. Bt11 event was the most used in GMM seeds, where seven out of eleven samples showed positive results. Two out of eleven hybrids showed the presence of the Bt176 event; however, MON810 not detected in any of the tested hybrids. We studied the Bt11 event in imported GMM seeds in Jordan for the first time, reinforcing the need for a mandatory labeling system and a valid simple qualitative method in routine analysis of GMCs.

这项研究旨在检测约旦生长的11种进口玉米杂交种种子中的转基因玉米（GMM）。我们使用启动子35 S和T-nos终止子对转基因材料进行常规筛选。常规PCR检测到用于筛选Bt 11，MON810和Bt176事件的特定事件。11个玉米杂种样品的种子对35 S启动子表现出阳性反应。十分之九的人对T-nos表示积极回应终结者。Bt11事件在GMM种子中使用最多，其中11个样本中有7个显示阳性结果。11个杂种中有2个显示出Bt176事件的存在；但是，在任何测试的混合动力车型中均未检测到MON810。我们首次在约旦进口GMM种子中研究了Bt11事件，从而增强了在常规GMC常规分析中对强制性标记系统和有效简单定性方法的需求。