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Molecular cloning and variant analysis of the TSHR gene in goose (Anser cygnoides).
British Poultry Science ( IF 1.6 ) Pub Date : 2020-05-18 , DOI: 10.1080/00071668.2020.1751803
C Wang 1 , Y Liu 1 , G Li 1 , S Gong 1 , Y Yang 1 , H Wang 1 , D He 1
Affiliation  

1. The thyroid-stimulating hormone receptor (TSHR) is a glycoprotein hormone receptor which has a pivotal role in metabolic regulation and photoperiod control during reproduction in birds and mammals. However, the molecular characterisation of TSHR in goose is unknown.

2. The goose TSHR cDNA (TSHR-1) is 2334 bp in length and encodes a protein of 763 amino acids. This trial identified another three novel splice variants of goose TSHR, TSHR-2 (lacking the exon 3 in TSHR-1 transcript), TSHR-3 (lacking the exon 6 in the TSHR-1 transcript) and TSHR-4 (lacking 12 bp of exon 8 and the entire exon 9 in the TSHR-1 transcript).

3. Bioinformatics analysis indicated that all the deduced TSHR amino acid sequences contained seven putative transmembrane domains, and the TSHR-3 protein lacked one potential N-linked glycosylation site (N-E-S) compared to the other three deduced proteins.

4. A phylogenetic tree based on amino acid sequences showed that the goose TSHR protein was closely related to those of other avian species, especially duck and chickens.

5. One microsatellite and three single nucleotide polymorphisms (SNPs) were identified. For the c1109A/G locus, AA and GA genotypes were found in the Zhedong-White goose population, GG and GA genotypes were detected in the Landes goose population, but the AA genotype was only detected in the other four goose populations.

6. All the information derived from this study can facilitate further studies on the functions of the goose TSHR gene.



中文翻译:

鹅(Anser cygnoides)TSHR基因的分子克隆和变异分析。

1. 促甲状腺激素受体(TSHR)是一种糖蛋白激素受体,在鸟类和哺乳动物繁殖过程中的代谢调节和光周期控制中起关键作用。然而,鹅中TSHR的分子特征尚不清楚。

2、鹅TSHR cDNA(TSHR-1)全长2334 bp,编码763个氨基酸的蛋白质。该试验确定了鹅TSHR 的另外三种新型剪接变体,TSHR-2(TSHR-1 转录本中缺少外显子 3)、TSHR-3(TSHR-1转录本中缺少外显子 6 )和TSHR-4(缺少 12 bp TSHR-1转录本中的外显子 8 和整个外显子 9 )。

3.生物信息学分析表明,所有推导的TSHR氨基酸序列均包含7个推定的跨膜结构域,与其他3个推导蛋白相比,TSHR-3蛋白缺乏一个潜在的N-连接糖基化位点(NES)。

4.基于氨基酸序列的系统发育树表明鹅TSHR蛋白与其他鸟类,尤其是鸭和鸡的亲缘关系密切。

5. 鉴定出1个微卫星和3个单核苷酸多态性(SNP)。对于c1109A/G位点,浙东-白鹅群体中检测到AA和GA基因型,兰德斯鹅群体中检测到GG和GA基因型,而仅在其他四个鹅群体中检测到AA基因型。

6、本研究获得的所有信息有助于进一步研究鹅TSHR基因的功能。

更新日期:2020-05-18
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