For Plasmodium falciparum related malaria (B50), one of the outstanding host factors for the development of severe disease is the ABO blood group of malaria patients, where blood group O reduces the probability of severe disease as compared to individuals of groups A, B, or AB. In this report, we investigate the stability of rosette aggregates in malaria caused by Plasmodium falciparum in microflows. These flows are created in microfluidic channels with stenosis-like constrictions of different widths down to ones narrower as the rosette's diameter. High speed videos were recorded and analyzed by a MATLAB© based tracking software (SURF: SUrvival of Rosettes in Flow). We find a correlation of rosette size, channel diameter, and blood group regarding the mobility of the rosettes. Following the concept of a thermodynamic model, we find a critical width of the stenosis for rosette rupture during their passage. Our data reveal that under physiologically relevant conditions, rosettes in blood group A have a higher rosette frequency and stability as compared to blood group O (BG O), which constitutes a crucial factor promoting the observed protection in BG O individuals against severe malaria in non-O individuals.

中文翻译：

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恶性疟原虫感染的毛细血管中血型和大小依赖性的稳定性。

对于恶性疟原虫相关的疟疾（B50），发生严重疾病的主要宿主因素之一是疟疾患者的ABO血型，其中血型O与A，B组相比降低了发生严重疾病的可能性，或AB。在此报告中，我们调查了微流中恶性疟原虫引起的疟疾中玫瑰花结聚集体的稳定性。这些流动是在微流体通道中形成的，狭窄程度如狭窄，狭窄程度随玫瑰花结直径而变窄。通过基于MATLAB©的跟踪软件（SURF：Rosettes in Flow的生存）来记录和分析高速视频。我们发现玫瑰花结的大小，通道直径和血型与玫瑰花结的流动性相关。遵循热力学模型的概念，我们发现瓣环在通过过程中狭窄的临界宽度。我们的数据显示，在生理相关条件下，与血型O（BG O）相比，血型A的玫瑰花结具有更高的玫瑰花频率和稳定性，这是促进观察到的BG O个体在非疟疾中预防严重疟疾的重要因素。 -O个人。