It has been shown that miR-144-3p regulates cell proliferation, apoptosis, migration and invasion in various cancers. However, the function and expression of miR-144-3p in colorectal cancer (CRC) remained obscure. Immunohistochemical (IHC) staining was performed to investigate the protein expression of BCL6 in CRC tissues. The effect of BCL6 and miR-144-3p on CRC cells was explored through methylthiazolyl tetrazolium (MTT) assay, colony formation and cell cycle assays. Luciferase reporter assays, reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot assay were carried out to determine that BCL6 is directly regulated by miR-144-3p. Our results showed that miR-144-3p is down-regulated in CRC and correlates with the tumor progression of CRC patients. miR-144-3p inhibits cell proliferation and delays G1/S phase transition of CRC cells. Moreover, we found that BCL6 is a new target of miR-144-3p. Furthermore, BCL6 is a mediator of miR-144-3p repression of cell proliferation and cell cycle arrest in CRC cells. miR-144-3p repression of Wnt/β-catenin signaling is mediated by BCL6 in CRC cells. Overall, the effect of the miR-144-3p/BCL6 axis on regulating CRC carcinogenesis was demonstrated, and miR-144-3p was identified as a potential prognostic and therapeutic target in colorectal cancer.

中文翻译：

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miR-144-3p 通过抑制 Wnt/β-catenin 信号传导靶向 BCL6 来抑制结直肠癌细胞的细胞增殖。

已显示 miR-144-3p 在各种癌症中调节细胞增殖、凋亡、迁移和侵袭。然而，miR-144-3p 在结直肠癌 (CRC) 中的功能和表达仍不清楚。进行免疫组织化学 (IHC) 染色以研究 BCL6 在 CRC 组织中的蛋白质表达。通过甲基噻唑基四唑 (MTT) 测定、集落形成和细胞周期测定探索 BCL6 和 miR-144-3p 对 CRC 细胞的影响。进行荧光素酶报告基因分析、逆转录定量聚合酶链反应 (RT-qPCR) 和蛋白质印迹分析以确定 BCL6 直接受 miR-144-3p 调节。我们的研究结果表明，miR-144-3p 在 CRC 中下调，并与 CRC 患者的肿瘤进展相关。miR-144-3p 抑制细胞增殖并延迟 CRC 细胞的 G1/S 期转变。此外，我们发现 BCL6 是 miR-144-3p 的新靶标。此外，BCL6 是 miR-144-3p 抑制 CRC 细胞中细胞增殖和细胞周期停滞的介质。miR-144-3p 对 Wnt/β-连环蛋白信号的抑制是由 CRC 细胞中的 BCL6 介导的。总体而言，证明了 miR-144-3p/BCL6 轴对调节 CRC 致癌作用的影响，并且 miR-144-3p 被确定为结直肠癌的潜在预后和治疗靶点。