Ultraviolet radiation induces biochemical and genetic changes in plants. The aim of this study was to investigate the effects of UV-B radiation on genetic stability, phenolic compounds and antioxidant activity of Pelargonium graveolens L′Her. Plant cuttings were exposed to 0, 0.12. 0.26 and 0.38 W/m² of UV-B radiation. Results indicated that by increasing the UV-B radiation intensity, total phenols, flavonoids and anthocyanin contents, Phenylalanine ammonia lyase activity and antioxidant capacity were increased. Analysis of four flavonols (quercetin, myricetin, kaempferol and rutin) contents of leaves extract by HPLC indicated that these four flavonols were enhanced in all treated plants and also the ratio of quercetin to kaempferol (Q/K) showed a significant increase (P ≤ 0.05) in UV-B treated plants in compare to control. To evaluate the genetic variation in treated plants, 10 ISSR primers were used. The highest level of percentage of polymorphism (P%), Shannon index (I), number of effective allele (Ne) and Nei’ genetic diversity (He), were observed at the highest UV-B radiation (0.38 W/m²). The AMOVA analysis also showed a significant genetic differentiation (P ≥ 0.001) among the studied groups, and confirmed the differentiation of groups obtained by the cluster analysis of molecular data. Overall, these results showed that biochemical changes in different intensities of UV-B were in line with genetic variations, so that the highest biochemical and genetic variations were observed in 0.38 W/m² treatment.

中文翻译：

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UV-B辐射对香叶天竺葵遗传和生化变化的影响。

紫外线辐射会诱发植物的生化和遗传变化。本研究的目的是研究UV-B辐射对天竺葵L'Her的遗传稳定性，酚类化合物和抗氧化活性的影响。将植物插条暴露于0、0.12。UV-B辐射为0.26和0.38 W / m ²。结果表明，通过增加UV-B辐射强度，总酚，类黄酮和花色苷含量，苯丙氨酸氨裂解酶活性和抗氧化能力得到提高。通过HPLC分析叶提取物中的四种黄酮醇（槲皮素，杨梅素，山奈酚和芦丁）含量表明，这四种黄酮醇在所有处理过的植物中均得到增强，并且槲皮素与山to酚的比率（Q / K）显示出显着增加（P 与对照相比，经UV-B处理的植株≤≤0.05）。为了评估处理植物的遗传变异，使用了10个ISSR引物。在最高的UV-B辐射（0.38 W / m ²）下观察到最高水平的多态性百分比（P％），香农指数（I），有效等位基因数量（Ne）和Nei'遗传多样性（He）。。的AMOVA分析还显示一个显著遗传分化（P  ≥0.001）所研究的群体中，并且证实了分子数据的聚类分析获得的基团的分化。总体而言，这些结果表明，不同强度的UV-B的生化变化与遗传变异一致，因此在0.38 W / m ^2的条件下观察到最高的生化和遗传变异。 治疗。