This study was conducted to evaluate the influence of brassinosteroid (24-epibrassinolide, EBL) seed priming and optimal nitrogen (N) supply in improving salt tolerance in soybean. The experimental treatments were (a) control (nutrient solution without N and without EBL priming), (b) nutrient solution without N and EBL seed priming, (c) N supplemented nutrient solution without EBL priming and (d) EBL seed priming + N supplemented nutrient solution under optimal (0 mM NaCl) and salt stress (0 mM NaCl) conditions. Salt stress caused significant reduction in growth and biomass accumulation of soybean. However, EBL seed priming and application of N improved the soybean performance under optimal and salt stress conditions. In this regard, treatments receiving both EBL and N were more effective. EBL priming and N, alone and in combination, triggered the accumulation of osmolytes including proline, glycine betaine and sugars resulting in better photo-protection through maintenance of tissue water content. Antioxidant activity and osmolyte accumulation significantly increased due to combined treatment of N and EBL under normal as well as salt stress conditions. In conclusion, salt stress caused reduction in growth and biomass soybean due to oxidative damage and osmotic stresses. However, soybean performance was improved by seed priming with EBL. Supplementation of N further improved the effectiveness of EBL treatment in improving salt tolerance in soybean.

中文翻译：

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含氮的油菜素类固醇种子引发可以提高大豆的耐盐性。

进行这项研究以评估油菜素甾醇（24-表油菜素内酯，EBL）种子引发和最佳氮（N）供应对提高大豆耐盐性的影响。实验处理方法是（a）对照（无氮且无EBL底漆的营养液），（b）无氮和EBL底漆的营养液，（c）无EBL底漆的N补充营养液和（d）EBL种子底漆+ N在最佳（0 mM NaCl）和盐胁迫（0 mM NaCl）条件下添加营养液。盐胁迫导致大豆生长和生物量积累显着下降。然而，在最佳和盐胁迫条件下，EBL种子引发和施用N可以改善大豆的性能。在这方面，同时接受EBL和N的治疗更为有效。EBL启动和N单独或组合使用时，触发了包括脯氨酸，甘氨酸甜菜碱和糖在内的渗透液的积累，从而通过维持组织中的水分而获得了更好的光保护作用。由于在正常和盐胁迫条件下对N和EBL的联合处理，抗氧化活性和渗透液的积累显着增加。总之，由于氧化损伤和渗透胁迫，盐胁迫导致大豆生长和生物量减少。但是，通过使用EBL进行底漆处理可改善大豆性能。补充氮进一步提高了EBL处理提高大豆耐盐性的有效性。由于在正常和盐胁迫条件下对N和EBL的联合处理，抗氧化活性和渗透压物质的积累显着增加。总之，由于氧化损伤和渗透胁迫，盐胁迫导致大豆生长和生物量减少。但是，通过使用EBL进行底漆处理可改善大豆性能。补充氮进一步提高了EBL处理提高大豆耐盐性的有效性。由于在正常和盐胁迫条件下对N和EBL的联合处理，抗氧化活性和渗透压物质的积累显着增加。总之，由于氧化损伤和渗透胁迫，盐胁迫导致大豆生长和生物量减少。但是，通过使用EBL进行底漆处理可改善大豆性能。补充氮进一步提高了EBL处理提高大豆耐盐性的有效性。