Abstract The details of how vegetation rehabilitation affects soil enzyme activities via changes of soil nutrients are unclear. To investigate how soil carbon and available nitrogen affect soil enzyme activities during the process of vegetation restoration, a factorial experiment was conducted with vegetation cover and nitrogen addition on degraded lands, and then a carbon addition experiment was performed with soil incubation. Soil enzyme activities were significantly higher under vegetation plots than those under bare plots and were highly related to the soil total carbon and nitrogen concentrations. For soils from bare plots, C addition increased β-1,4-glucosidase (BG) activities, β-N-acetyl glucosaminidase (NAG) activities, leucine aminopeptidase (LAP) activities and acid phosphomonoesterase (AP) activities by 93%, 784%, 268% and 272%, respectively. By comparision, C addition only increased activities of BG, NAG, LAP and AP by 6%, 54%, 45% and 214% for soils from vegetation plots, respectively. Furthermore, the interaction between C and N significantly affected the activities of AP and BG. These findings indicate that the elevation of enzyme activities during vegetation rehabilitation is mainly driven by the relaxation of C–N limitation for microorganisms.

中文翻译：

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植被恢复通过碳供应和氮保留增加退化土地的土壤酶活性

摘要 植被恢复如何通过土壤养分的变化影响土壤酶活性的细节尚不清楚。为研究植被恢复过程中土壤碳和速效氮对土壤酶活性的影响，在退化土地上进行了植被覆盖和氮添加的因子试验，然后进行了土壤孵化的碳添加试验。植被样地土壤酶活性显着高于裸地土壤酶活性，且与土壤总碳、氮浓度高度相关。对于来自裸地的土壤，添加 C 使 β-1,4-葡萄糖苷酶 (BG) 活性、β-N-乙酰氨基葡萄糖苷酶 (NAG) 活性、亮氨酸氨基肽酶 (LAP) 活性和酸性磷酸单酯酶 (AP) 活性增加了 93%，784 %、268% 和 272%，分别。相比之下，添加碳仅使植被样地土壤的 BG、NAG、LAP 和 AP 的活性分别增加了 6%、54%、45% 和 214%。此外，C和N之间的相互作用显着影响AP和BG的活性。这些发现表明，植被恢复过程中酶活性的提高主要是由微生物 C-N 限制的放松驱动的。