Kinesin-7 CENP-E regulates chromosome alignment and genome stability of spermatogenic cells.,Cell Death Discovery

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Kinesin-7 CENP-E regulates chromosome alignment and genome stability of spermatogenic cells.
Cell Death Discovery ( IF 6.1 ) Pub Date : 2020-04-20 , DOI: 10.1038/s41420-020-0261-8
Zhen-Yu She _{1,

2} , Kai-Wei Yu _{1,

2} , Ning Zhong _{1,

2} , Yu Xiao _{1,

2} , Ya-Lan Wei _{3,

4} , Yang Lin _{1,

2} , Yue-Ling Li _{1,

2} , Ming-Hui Lu _{1,

2}

Affiliation

Kinesin-7 CENP-E is an essential kinetochore motor required for chromosome alignment and congression. However, the specific functions of CENP-E in the spermatogenic cells during spermatogenesis remain unknown. In this study, we find that CENP-E proteins are expressed in the spermatogonia, spermatocytes, and the elongating spermatids. CENP-E inhibition by specific inhibitor GSK923295 results in the disruption of spermatogenesis and cell cycle arrest of spermatogenic cells. Both spermatogonia and spermatocytes are arrested in metaphase and several chromosomes are not aligned at the equatorial plate. We find that CENP-E inhibition leads to chromosome misalignment, the spindle disorganization, and the formation of the aneuploidy cells. Furthermore, the inhibition of CENP-E results in the defects in the formation of spermatids, including the sperm head condensation and the sperm tail formation. We have revealed that kinesin-7 CENP-E is essential for chromosome alignment and genome stability of the spermatogenic cells.

中文翻译：

Kinesin-7 CENP-E 调节生精细胞的染色体排列和基因组稳定性。

Kinesin-7 CENP-E 是染色体排列和国会所需的重要动粒马达。然而，CENP-E在精子发生过程中在生精细胞中的具体功能仍不清楚。在这项研究中，我们发现CENP-E蛋白在精原细胞、精母细胞和伸长的精子细胞中表达。特异性抑制剂 GSK923295 抑制 CENP-E 会导致精子发生中断和生精细胞的细胞周期停滞。精原细胞和精母细胞都停滞在中期，并且一些染色体在赤道板处不对齐。我们发现 CENP-E 抑制会导致染色体错位、纺锤体解体和非整倍体细胞的形成。此外，CENP-E的抑制会导致精子形成的缺陷，包括精子头部的凝结和精子尾部的形成。我们发现驱动蛋白 7 CENP-E 对于生精细胞的染色体排列和基因组稳定性至关重要。

更新日期：2020-04-24

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