Ultrabright fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays.,Nature Biomedical Engineering

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Ultrabright fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays.
Nature Biomedical Engineering ( IF 26.8 ) Pub Date : 2020-04-20 , DOI: 10.1038/s41551-020-0547-4
Jingyi Luan ₁ , Anushree Seth ₁ , Rohit Gupta ₁ , Zheyu Wang ₁ , Priya Rathi ₁ , Sisi Cao ₁ , Hamed Gholami Derami ₁ , Rui Tang ₂ , Baogang Xu ₂ , Samuel Achilefu ₂ , Jeremiah J Morrissey _{3,

4} , Srikanth Singamaneni _{1,

4}

Affiliation

The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging. Here, we show that a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices. The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element. Its emission wavelength can be tuned over the visible and near-infrared spectral regions by modifying its size, shape and composition. It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.

中文翻译：

超亮荧光纳米级标记，用于通过标准生物测定法在飞克分子中检测分析物。

生物样品中低丰度分子生物标志物的检测和定量具有挑战性。在这里，我们表明，等离子纳米级构建体可作为多种生物测定的“附加”标签，可改善其信噪比和动态范围，而无需更改其工作流程和读出装置。等离子体激元构建体由牛血清白蛋白支架和约210个IRDye 800CW荧光团（荧光强度约为单个800CW荧光团的荧光强度的6700倍），聚合物涂层的金纳米棒充当等离子天线以及生物素作为高亲和力生物识别元件。通过改变其大小，形状和成分，可以在可见光和近红外光谱区域内调节其发射波长。

更新日期：2020-04-24

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