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Temporal changes in microbial communities attached to forages with different lignocellulosic compositions in cattle rumen.
FEMS Microbiology Ecology ( IF 3.5 ) Pub Date : 2020-06-01 , DOI: 10.1093/femsec/fiaa069
Javad Gharechahi 1 , Mohammad Farhad Vahidi 2 , Xue-Zhi Ding 3 , Jian-Lin Han 4, 5 , Ghasem Hosseini Salekdeh 2, 6
Affiliation  

The attachment of rumen microbes to feed particles is critical to feed fermentation, degradation and digestion. However, the extent to which the physicochemical properties of feeds influence the colonization by rumen microbes is still unclear. We hypothesized that rumen microbial communities may have differential preferences for attachments to feeds with varying lignocellulose properties. To this end, the structure and composition of microbial communities attached to six common forages with different lignocellulosic compositions were analyzed following in situ rumen incubation in male Taleshi cattle. The results showed that differences in lignocellulosic compositions significantly affected the inter-sample diversity of forage-attached microbial communities in the first 24 h of rumen incubation, during which the highest dry matter degradation was achieved. However, extension of the incubation to 96 h resulted in the development of more uniform microbial communities across the forages. Fibrobacteres were significantly overrepresented in the bacterial communities attached to the forages with the highest neutral detergent fiber contents. Ruminococcus tended to attach to the forages with low acid detergent lignin contents. The extent of dry matter fermentation was significantly correlated with the populations of Fibrobacteraceae, unclassified Bacteroidales, Ruminococcaceae and Spirochaetacea. Our findings suggested that lignocellulosic compositions, and more specifically the cellulose components, significantly affected the microbial attachment to and thus the final digestion of the forages.

中文翻译:

牛瘤胃中具有不同木质纤维素成分的饲料所附着的微生物群落的时间变化。

瘤胃微生物与饲料颗粒的附着对饲料的发酵,降解和消化至关重要。但是,饲料的理化性质影响瘤胃微生物定殖的程度仍不清楚。我们假设瘤胃微生物群落对木质纤维素特性不同的饲料的附着可能有不同的偏好。为此,在雄性塔勒西牛原位瘤胃温育之后,分析了附着于具有不同木质纤维素成分的六个普通草料上的微生物群落的结构和组成。结果表明,在瘤胃孵化的最初24小时内,木质纤维素成分的差异会显着影响附着于饲草的微生物群落的样品间多样性,在此期间,干物质降解最高。但是,将培养时间延长至96小时导致整个草料中形成更统一的微生物群落。在附着于具有最高中性洗涤剂纤维含量的饲草的细菌群落中,纤维杆菌明显过量存在。瘤胃球菌倾向于以低酸洗涤剂木质素含量附着在草料上。干物质发酵的程度与纤维杆菌科,未分类的拟杆菌科,Ruminococcaceae和Spirochaetacea的种群显着相关。我们的发现表明,木质纤维素组合物,更具体地说是纤维素组分,显着影响了微生物对草料的附着,从而最终影响了草料的最终消化。孵育时间延长至96小时导致整个草料中形成更统一的微生物群落。在附着于具有最高中性洗涤剂纤维含量的饲草的细菌群落中,纤维杆菌明显过量存在。瘤胃球菌倾向于以低酸洗涤剂木质素含量附着在草料上。干物质发酵的程度与纤维杆菌科,未分类的拟杆菌科,Ruminococcaceae和Spirochaetacea的种群显着相关。我们的发现表明,木质纤维素组合物,更具体地说是纤维素组分,显着影响了微生物对草料的附着,从而最终影响了草料的最终消化。孵育时间延长至96小时导致整个草料中形成更统一的微生物群落。在附着于具有最高中性洗涤剂纤维含量的饲草的细菌群落中,纤维杆菌明显过量存在。瘤胃球菌倾向于以低酸洗涤剂木质素含量附着在草料上。干物质发酵的程度与纤维杆菌科,未分类的拟杆菌科,Ruminococcaceae和Spirochaetacea的种群显着相关。我们的发现表明,木质纤维素组合物,更具体地说是纤维素组分,显着影响了微生物对草料的附着,从而最终影响了草料的最终消化。在附着于具有最高中性洗涤剂纤维含量的饲草的细菌群落中,纤维杆菌明显过量存在。瘤胃球菌倾向于以低酸洗涤剂木质素含量附着在草料上。干物质发酵的程度与纤维杆菌科,未分类的拟杆菌科,Ruminococcaceae和Spirochaetacea的种群显着相关。我们的发现表明,木质纤维素组合物,尤其是纤维素组分,显着影响了微生物对草料的附着,从而最终影响了草料的最终消化。在附着于具有最高中性洗涤剂纤维含量的草料的细菌群落中,纤维菌明显过量存在。瘤胃球菌倾向于以低酸洗涤剂木质素含量附着在草料上。干物质发酵的程度与纤维杆菌科,未分类的拟杆菌科,Ruminococcaceae和Spirochaetacea的种群显着相关。我们的发现表明,木质纤维素组合物,更具体地说是纤维素组分,显着影响了微生物对草料的附着,从而最终影响了草料的最终消化。干物质发酵的程度与纤维杆菌科,未分类的细菌科,Ruminococcaceae和Spirochaetacea的种群显着相关。我们的发现表明,木质纤维素组合物,尤其是纤维素组分,显着影响了微生物对草料的附着,从而最终影响了草料的最终消化。干物质发酵的程度与纤维杆菌科,未分类的细菌科,Ruminococcaceae和Spirochaetacea的种群显着相关。我们的发现表明,木质纤维素组合物,尤其是纤维素组分,显着影响了微生物对草料的附着,从而最终影响了草料的最终消化。
更新日期:2020-04-18
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