Wound healing involves the concerted action of various lymphoid and in particular myeloid cell populations. To characterize and quantitate different types of myeloid cells and to obtain information on their kinetics during wound healing, we performed multiparametric flow cytometry analysis. In healthy mice, neutrophil numbers increased early after injury and returned to near basal levels after completion of healing. Macrophages, monocyte‐derived dendritic cells (DCs), and eosinophils were abundant throughout the healing phase, in particular in early wounds, and Langerhans cells increased after wounding and remained elevated after epithelial closure. Major differences in healing‐impaired diabetic mice were a much higher percentage of immune cells in late wounds, mainly as a result of neutrophil, macrophage, and monocyte persistence; reduced numbers and percentages of macrophages and monocyte‐derived DCs in early wounds; and of Langerhans cells, conventional DCs, and eosinophils throughout the healing process. Finally, unbiased cluster analysis (PhenoGraph) identified a large number of different clusters of myeloid cells in skin wounds. These results provide insight into myeloid cell diversity and dynamics during wound repair and highlight the abnormal inflammatory response associated with impaired healing.

中文翻译：

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在健康的和受损的糖尿病小鼠伤口愈合过程中，髓样细胞的全面表征。

伤口愈合涉及各种淋巴样，尤其是髓样细胞群体的协同作用。为了表征和定量不同类型的骨髓细胞并获得伤口愈合过程中的动力学信息，我们进行了多参数流式细胞仪分析。在健康小鼠中，中性粒细胞数量在损伤后早期增加，并在愈合完成后恢复至接近基础水平。在整个愈合阶段，尤其是在早期伤口中，巨噬细胞，单核细胞衍生的树突状细胞（DCs）和嗜酸性粒细胞丰富，而Langerhans细胞在受伤后增加，并在上皮闭合后保持升高。康复受损的糖尿病小鼠的主要差异是晚期伤口的免疫细胞百分比高得多，这主要是由于中性粒细胞，巨噬细胞和单核细胞的持久性所致。减少早期伤口中巨噬细胞和单核细胞衍生DC的数量和百分比；在整个愈合过程中，还有朗格汉斯细胞，常规DC和嗜酸性粒细胞。最后，无偏聚类分析（PhenoGraph）确定了皮肤伤口中大量不同的髓样细胞簇。这些结果提供了对伤口修复过程中骨髓细胞多样性和动力学的了解，并突出了与愈合受损相关的异常炎症反应。