The transgene‐directed accumulation of non‐native omega‐3 long chain polyunsaturated fatty acids in the seed oil of Camelina sativa (Camelina) was evaluated in the field, in distinct geographical and regulatory locations. A construct, DHA2015.1, containing an optimal combination of biosynthetic genes, was selected for experimental field release in the UK, USA and Canada, and the accumulation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) determined. The occurrence of these fatty acids in different triacylglycerol species was monitored and found to follow a broad trend irrespective of the agricultural environment. This is a clear demonstration of the stability and robust nature of the transgenic trait for omega‐3 long chain polyunsaturated fatty acids in Camelina. Examination of non‐seed tissues for the unintended accumulation of EPA and DHA failed to identify their presence in leaf, stem, flower, anther or capsule shell material, confirming the seed‐specific accumulation of these novel fatty acids. Collectively, these data confirm the promise of GM plant‐based sources of so‐called omega‐3 fish oils as a sustainable replacement for oceanically derived oils.

中文翻译：

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田间种植的转基因骆驼科植物中EPA和DHA的高水平积累-TAG积累和异质性的多领域评估。

苜蓿种子油中非天然omega-3长链多不饱和脂肪酸的转基因定向积累（Camelina）在不同的地理和法规位置进行了现场评估。选择了包含生物合成基因最佳组合的构建体DHA2015.1在英国，美国和加拿大进行实验田间释放，并测定了二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的积累。监测了这些脂肪酸在不同的三酰基甘油物种中的出现，并且发现其遵循广泛的趋势，而与农业环境无关。这清楚地证明了卡梅利纳（Camelina）中omega-3长链多不饱和脂肪酸转基因性状的稳定性和鲁棒性。检查非种子组织中是否存在EPA和DHA的意外积累，未能发现它们在叶，茎，花，花药或胶囊壳材料中的存在，证实了这些新型脂肪酸的种子特异性积累。总体而言，这些数据证实了基于转基因植物的所谓omega-3鱼油来源有望作为海洋衍生油的可持续替代品。