Molecular detection of sugarcane striate virus and sugarcane white streak virus and their prevalence in the Miami World Collection of sugarcane and related grasses,Plant Pathology

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Molecular detection of sugarcane striate virus and sugarcane white streak virus and their prevalence in the Miami World Collection of sugarcane and related grasses
Plant Pathology ( IF 2.3 ) Pub Date : 2020-05-22 , DOI: 10.1111/ppa.13192
Wardatou Boukari ₁ , Denis Filloux _{2,

3} , Jean Heinrich Daugrois _{2,

3} , Emmanuel Fernandez _{2,

3} , Dimitre Mollov ₄ , Claudia Kaye ₅ , Martha Hincapie ₁ , Alexander Sanchez ₆ , Jianping Wang ₇ , Philippe Roumagnac _{2,

3} , Philippe Rott _{1,

2,

3}

Affiliation

Viruses in the genus Mastrevirus (family Geminiviridae ), including those infecting sugarcane, have natural geographical ranges almost exclusively restricted to Africa and the Indian Ocean islands off the African coast. Only sugarcane white streak virus (SWSV) in Barbados and sugarcane striate virus (SStrV) in Florida and Guadeloupe are known to infect a few sugarcane varieties in the Western Hemisphere. In this study, PCR assays were developed to detect these two viruses in sugarcane. Five hundred and seventy‐one DNA samples from Saccharum species and interspecific hybrids from the Miami World Collection of sugarcane and related grasses were tested for the presence of SStrV and SWSV by PCR. No variety was found infected by SWSV but SStrV was detected in 19 varieties. PCR data were confirmed by sequencing amplified fragments (248 bp). These fragments shared 93%–100% nucleotide identity with SStrV sequences from the GenBank database. SStrV isolates were distributed in six phylogenetic groups, including the four strains of the virus. Most varieties infected by SStrV originated from Asia, thus confirming a previous hypothesis stating that this virus originated from this continent. Absence of SStrV in commercial sugarcane in Florida also suggested that this virus has not been spread in this location, while infected plants have been present for several decades.

中文翻译：

甘蔗条纹病毒和甘蔗白条病毒的分子检测及其在迈阿密世界甘蔗及相关草种收藏中的流行

Mastrevirus 属（双生病毒科）中的病毒，包括那些感染甘蔗的病毒，其自然地理范围几乎仅限于非洲和非洲海岸附近的印度洋岛屿。已知只有巴巴多斯的甘蔗白色条纹病毒 (SWSV) 和佛罗里达州和瓜德罗普岛的甘蔗条纹病毒 (SStrV) 会感染西半球的一些甘蔗品种。在这项研究中，开发了 PCR 分析来检测甘蔗中的这两种病毒。通过 PCR 测试了来自甘蔗和相关草的迈阿密世界收藏的甘蔗物种和种间杂种的 571 个 DNA 样本中 SStrV 和 SWSV 的存在。未发现品种感染 SWSV，但在 19 个品种中检出 SStrV。PCR 数据通过对扩增片段 (248 bp) 进行测序来确认。这些片段与 GenBank 数据库中的 SStrV 序列具有 93%–100% 的核苷酸同一性。SStrV 分离株分布在六个系统发育组中，包括四种病毒株。大多数被 SStrV 感染的品种起源于亚洲，从而证实了先前的假设，即该病毒起源于这个大陆。佛罗里达州商业甘蔗中不存在 SStrV 也表明该病毒尚未在该地区传播，而受感染的植物已存在数十年。因此证实了先前的假设，即该病毒起源于该大陆。佛罗里达州商业甘蔗中不存在 SStrV 也表明该病毒尚未在该地区传播，而受感染的植物已存在数十年。因此证实了先前的假设，即该病毒起源于该大陆。佛罗里达州商业甘蔗中不存在 SStrV 也表明该病毒尚未在该地区传播，而受感染的植物已存在数十年。

更新日期：2020-05-22

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