Severe Acute Respiratory Syndrome coronavirus 2 (SARS‐CoV‐2) is rapidly spreading around the world. There is no existing vaccine or proven drug to prevent infections and stop virus proliferation. Although this virus is similar to human and animal SARS‐CoVs and Middle East Respiratory Syndrome coronavirus (MERS‐CoVs), the detailed information about SARS‐CoV‐2 proteins structures and functions is urgently needed to rapidly develop effective vaccines, antibodies, and antivirals. We applied high‐throughput protein production and structure determination pipeline at the Center for Structural Genomics of Infectious Diseases to produce SARS‐CoV‐2 proteins and structures. Here we report two high‐resolution crystal structures of endoribonuclease Nsp15/NendoU. We compare these structures with previously reported homologs from SARS and MERS coronaviruses.

中文翻译：

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来自 SARS-CoV-2 的 Nsp15 核糖核酸内切酶 NendoU 的晶体结构。

严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 正在世界各地迅速传播。没有现有的疫苗或经过验证的药物可以预防感染和阻止病毒增殖。尽管该病毒与人和动物的 SARS-CoV 和中东呼吸综合征冠状病毒 (MERS-CoV) 相似，但迫切需要有关 SARS-CoV-2 蛋白质结构和功能的详细信息，以快速开发有效的疫苗、抗体和抗病毒药物. 我们在传染病结构基因组学中心应用高通量蛋白质生产和结构确定流程来生产 SARS-CoV-2 蛋白质和结构。在这里，我们报告了核糖核酸内切酶 Nsp15/NendoU 的两种高分辨率晶体结构。我们将这些结构与先前报道的 SARS 和 MERS 冠状病毒同源物进行比较。