Highbush blueberry ( Vaccinium corymbosum L.) is beneficial for human health due to its bioactive properties. Agronomic management includes boron (B) addition to promote its growth and development. We aimed to provide new insights into the effects of B supply on the phenolic profile and antioxidative capacity in the leaves of two common cultivars, Brigitta and Legacy. Seedlings of Brigitta and Legacy were supplied with different B concentrations (0–50 mg B L −1 ). After 30 days, leaf biomass and B content, the activity of phenylalanine-ammonia-lyase (PAL, EC 4.3.1.24), the phenolic profile, and radical scavenging activity of methanolic extracts (oxygen radical absorbance capacity (ORAC), reducing 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing-antioxidant power (FRAP)) were analyzed. Tolerance to B was assessed by the lipid peroxidation level and superoxide dismutase (SOD, EC 1.15.1.1) activity. Leaf biomass in cultivars increased at 3 mg B L −1 , while Legacy was more sensitive to higher B treatments than Brigitta, which was evidenced by increased lipid peroxidation and decreased SOD activity. Boron-induced inhibition of PAL activity was higher in Legacy. Cultivar-specific differences observed in hydroxycinnamic, flavonoid, and anthocyanin profiles of leaf extracts were related to radical scavenging capacities. The results indicate different B requirements in the two blueberry genotypes, which should be considered before B application to obtain an optimal antioxidant composition. Boron supply increased the accumulation of flavonoids in Brigitta and hydroxycinnamates in Legacy. Higher tolerance to excess B in Brigitta may be related to higher antioxidative capacity and SOD activity than Legacy.

中文翻译：

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两种高丛蓝莓基因型对硼供应的差异耐受性和酚类叶型

Highbush 蓝莓 ( Vaccinium corymbosum L.) 由于其生物活性特性而对人类健康有益。农艺管理包括添加硼（B）以促进其生长发育。我们旨在提供有关 B 供应对两种常见品种 Brigitta 和 Legacy 叶片酚类成分和抗氧化能力影响的新见解。Brigitta 和 Legacy 的幼苗提供不同的 B 浓度 (0–50 mg BL -1 )。30 天后，叶片生物量和 B 含量、苯丙氨酸-氨裂解酶 (PAL, EC 4.3.1.24) 的活性、酚类成分和甲醇提取物的自由基清除活性（氧自由基吸收能力 (ORAC)，减少 2，分析了 2-diphenyl-1-picrylhydrazyl (DPPH) 和铁还原抗氧化能力 (FRAP)。通过脂质过氧化水平和超氧化物歧化酶 (SOD, EC 1.15.1.1) 活性评估对 B 的耐受性。栽培品种中的叶生物量在 3 mg BL -1 时增加，而 Legacy 对高 B 处理比 Brigitta 更敏感，这可以通过脂质过氧化增加和 SOD 活性降低来证明。硼诱导的 PAL 活性抑制在 Legacy 中更高。在叶提取物的羟基肉桂酸、类黄酮和花青素谱中观察到的品种特异性差异与自由基清除能力有关。结果表明，两种蓝莓基因型对 B 的需求不同，在施用 B 之前应考虑这一点，以获得最佳的抗氧化剂组合物。硼供应增加了 Brigitta 中类黄酮和 Legacy 中羟基肉桂酸酯的积累。