Avermectin (AVM) has been widely used in agriculture and animal husbandry for the treatment of broad spectrum parasitic diseases, but it has a harmful effect on human body through food chain. In this paper, on the basis of anti-AVM specific antibody, expanded mesoporous silica-encapsulated gold nanoparticles (EMSN-AuNPs)-based enzyme-linked immunosorbent assay (ELISA) for the detection of AVM was developed. Under the optimized conditions, the linear range of the developed EMSN-AuNPs-based ELISA was from 2.53 to 184.28 ng/mL, with the limit of detection (LOD) of 2.17 ng/mL. In addition, the cross-reactivity to macrolide antibiotic analogues was less than 0.1%. Therefore, the established EMSN-AuNPs-based ELISA was used to detect the AVM residues in the spiked samples, with the recovery from 99.5 to 101.5%, and the results was further validated by high-performance liquid chromatography (HPLC) and ELISA. Therefore, the constructed EMSN-AuNPs-based ELISA is suitable for monitoring AVM in biological samples with its sensitivity, accuracy, and real-timing.

Graphical Abstract

阿维菌素（AVM）已广泛用于农业和畜牧业，用于治疗广谱性寄生虫病，但它通过食物链对人体有害。本文在抗AVM特异性抗体的基础上，开发了基于介孔二氧化硅包裹的金纳米颗粒（EMSN-AuNPs）的酶联免疫吸附试验（ELISA），用于检测AVM。在优化的条件下，已开发的基于EMSN-AuNPs的ELISA的线性范围为2.53至184.28 ng / mL，检测限（LOD）为2.17 ng / mL。另外，与大环内酯类抗生素类似物的交叉反应性小于0.1％。因此，已建立的基于EMSN-AuNPs的ELISA用于检测加标样品中的AVM残留，回收率从99.5％提高到101.5％，并通过高效液相色谱（HPLC）和ELISA进一步验证了结果。因此，构建的基于EMSN-AuNPs的ELISA具有灵敏性，准确性和实时性，适用于监测生物样品中的AVM。

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