Syndecans (SDC) are important multifunctional components of the extracellular matrix mainly described in endothelial cells. We studied the expression and regulation of SDC in cultured MIN6B1 cells and pancreatic islets. qRT-PCR revealed that syndecan-4 (SDC4) was the predominant isoform expressed in MIN6B1 cells and islets compared to other forms of SDC. Immunofluorescence in mouse and human pancreas sections revealed that SDC4 is mainly expressed in β-cells compared to other pancreatic cells. Exposure of MIN6B1 and human islets to IL-1β dose-dependently induced a rapid and transient expression of SDC4 while SRC and STAT3 inhibitors decreased this effect. Exposure of human islets to Il-1β caused an increase of SDC4 shedding, however treatment with STAT3 and SRC inhibitors inhibited this effect. These results indicate that SDC4 is upregulated by IL-1β through the SRC-STAT3 pathway and this pathway is also involved in SDC4 shedding in islets.

中文翻译：

---

Syndecan-4在β细胞和人类胰岛中受到IL-1β的调节。

Syndecans（SDC）是主要描述于内皮细胞中的细胞外基质的重要多功能组分。我们研究了SDC在培养的MIN6B1细胞和胰岛中的表达和调控。qRT-PCR显示，与其他形式的SDC相比，syndecan-4（SDC4）是MIN6B1细胞和胰岛中表达的主要同工型。小鼠和人胰腺切片中的免疫荧光显示，与其他胰腺细胞相比，SDC4主要在β细胞中表达。MIN6B1和人类胰岛暴露于IL-1β剂量依赖性诱导SDC4的快速和瞬时表达，而SRC和STAT3抑制剂则降低了这种作用。人类胰岛暴露于II-1β导致SDC4脱落增加，但是用STAT3和SRC抑制剂治疗抑制了这一作用。