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Development of a 64 SNV panel for breed authentication in Iberian pigs and their derived meat products.
Meat Science ( IF 5.7 ) Pub Date : 2020-04-19 , DOI: 10.1016/j.meatsci.2020.108152
M Muñoz 1 , J M García-Casco 1 , E Alves 2 , R Benítez 2 , C Barragán 2 , C Caraballo 3 , A I Fernández 2 , F García 2 , Y Núñez 2 , C Óvilo 2 , A Fernández 2 , C Rodríguez 2 , L Silió 2
Affiliation  

Spanish legislation regulates the labelling of Iberian pig meat and dry-cured products, which are labelled as “Ibérico” or “100% Ibérico” when they come from Duroc x Iberian crossbred or Iberian purebred pigs. Although the analytical authentication of breed origin is not mandatory, a genetic diagnostic tool is demanded by producers and consumers. We have designed a 64 Single Nucleotide Variant genotyping panel displaying extreme allelic frequencies between Duroc and Iberian purebred samples. Average proportions of Iberian alleles of 0.99, 0.01, 0.77 and 0.48 were estimated by admixture clustering analysis of known origin samples, for Iberian and Duroc purebred, 75% Iberian and 50% Iberian classes, respectively. A supervised analysis with 1419 samples showed some overlapping between contiguous classes, but the calculated degrees of separability ranged from 0.800 to 0.996, exceeding the threshold value (0.70) for considering suitable for prediction. Therefore, this panel is a useful genetic tool to infer purebred or crossbred Iberian origin of live animals, meat and dry-cured products.



中文翻译:

开发了一个64 SNV面板,用于伊比利亚猪及其衍生肉制品的品种鉴定。

西班牙立法对伊比利亚猪肉和干腌产品的标签进行了规定,当它们来自杜洛克x伊比利亚杂交或伊比利亚纯种猪时,被标记为“伊比利亚”或“ 100%伊比利亚”。尽管对品种起源的分析鉴定不是强制性的,但生产者和消费者都需要一种遗传诊断工具。我们设计了64个单核苷酸变异基因分型面板,显示杜洛克和伊比利亚纯种样品之间的极端等位基因频率。通过已知来源样品的混合聚类分析,分别估计了伊比利亚和杜洛克纯种,伊比利亚75%和伊比利亚50%类别的伊比利亚等位基因的平均比例为0.99、0.01、0.77和0.48。在对1419个样本的监督分析中,连续的类之间存在一些重叠,但计算得出的可分离度在0.800至0.996的范围内,超过了认为适合预测的阈值(0.70)。因此,该小组是推断活体动物,肉和干腌制品的纯种或杂种伊比利亚起源的有用的遗传工具。

更新日期:2020-04-19
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