Numerous value-added chemicals can be produced using xylan as a feedstock. However, the product yields are limited by low xylan utilization efficiency, as well as by carbon flux competition between biomass production and biosynthesis. Herein, a dynamic consolidated bioprocessing strategy was developed, which coupled xylan utilization and yield optimization modules. Specifically, we achieved the efficient conversion of xylan to valuable chemicals in a fully consolidated manner by optimizing the expression level of xylanases and xylose transporter in the xylan utilization module. Moreover, a cell density-dependent, and Cre-triggered dynamic system that enabled the dynamic decoupling of biosynthesis and biomass production was constructed in the yield optimization module. The final shake flask-scale titers of xylonate, produced through an exogenous pathway, and shikimate, produced through an endogenous pathway, reached 16.85 and 3.2 g L⁻¹, respectively. This study not only provides an efficient microbial platform for the utilization of xylan, but also opens up the possibility for the large-scale production of high value-added chemicals from renewable feedstocks.

使用木聚糖作为原料可以生产许多增值化学品。然而，产物产量受到木聚糖利用效率低以及生物质生产与生物合成之间的碳通量竞争的限制。本文中，开发了一种动态合并生物处理策略，该策略结合了木聚糖利用和产量优化模块。具体而言，我们通过优化木聚糖利用模块中的木聚糖酶和木糖转运蛋白的表达水平，以完全整合的方式实现了木聚糖有效转化为有价值的化学物质。此外，在产量优化模块中构建了依赖于细胞密度的Cre触发动态系统，该系统能够实现生物合成与生物量生产的动态解耦。通过外源途径产生的木糖酸盐和通过内源途径产生的sh草酸盐的最终摇瓶规模滴度分别达到16.85和3.2g L ^-1。这项研究不仅为木聚糖的利用提供了一个有效的微生物平台，而且还为利用可再生原料大规模生产高附加值化学品提供了可能性。