Cell mediated immunity of the honey bee (Apis mellifera) involves the activity of several hemocyte populations, currently defined by morphological features and lectin binding characteristics. The objective of the present study was to identify molecular markers capable of characterizing subsets of honey bee hemocytes. We developed and employed monoclonal antibodies with restricted reactions to functionally distinct hemocyte subpopulations. Melanizing cells, known as oenocytoids, were defined by an antibody to prophenoloxidase, aggregating cells were identified by the expression of Hemolectin, and phagocytic cells were identified by a marker expressed on granulocytes. We anticipate that this combination of antibodies not only allows for the detection of functionally distinct hemocyte subtypes, but will help to further the exploration of hematopoietic compartments, as well as reveal details of the honey bee cellular immune defense against parasites and microbes.

中文翻译：

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鉴定用于表征蜜蜂（Apis mellifera）血细胞类别的参考标记。

蜜蜂（Apis mellifera）的细胞介导免疫涉及几种血细胞群的活动，目前由形态特征和凝集素结合特征定义。本研究的目的是确定能够表征蜜蜂血细胞亚群的分子标记。我们开发并使用了对功能不同的血细胞亚群反应受限的单克隆抗体。黑色素细胞，称为卵胞苷，由酚氧化酶原抗体定义，聚集细胞由血凝素的表达识别，吞噬细胞由粒细胞上表达的标记物识别。我们预计这种抗体组合不仅可以检测功能不同的血细胞亚型，