Halomonas TD01, which can grow under non-sterile and continuous processes at high pH and high salt concentrations, is a robust platform for PHA production from glucose. For extending other low-cost sustainable substrates and increasing the potential application in other value-added products, a better understanding of substrates utilization and chemicals tolerance is necessary. In this study, the substrate profiling of TD01 was analyzed via Biolog. Phenotype microarray results demonstrated that TD01 has a wide-ranging substrate spectrum and can utilize 140 of the 190 test compounds. Some cheap, abundant carbon sources, such as sodium acetate, glycerol, ethanol and lactate can well support the growth of TD01 in shake-flask, and are therefore suggested to be its alternative low-cost substrates for chemicals production in future. Furthermore, the tolerance of TD01 to various chemicals was tested. The results showed that the tolerability of TD01 to high concentrations of organic acid salts is prominent. When adding 75 g/L sodium acetate, 100 g/L succinic acid and 100 g/L itaconic acid in the medium, the growth rate reduced 56.14%, 52.63% and 47.37%, respectively. All these results highlight TD01 as a promising, next generation industrial workhorse in chemicals biomanufacturing from cheap organic acid salts.

中文翻译：

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Halomonas TD01的底物分析和公差测试表明其在化学物质可持续生产中的潜在应用。

Halomonas TD01可在高pH和高盐浓度的非无菌和连续过程中生长，是从葡萄糖生产PHA的强大平台。为了扩展其他低成本的可持续性基材并增加在其他增值产品中的潜在应用，必须更好地了解基材的利用率和耐化学药品性。在这项研究中，通过Biolog分析了TD01的底物谱。表型微阵列结果表明，TD01具有广泛的底物谱，可以利用190种测试化合物中的140种。一些便宜而丰富的碳源，例如乙酸钠，甘油，乙醇和乳酸盐，可以很好地支持TD01在摇瓶中的生长，因此建议将其作为未来化学品生产的低成本低成本底物。此外，测试了TD01对各种化学品的耐受性。结果表明，TD01对高浓度有机酸盐的耐受性是突出的。在培养基中添加75 g / L乙酸钠，100 g / L琥珀酸和100 g / L衣康酸时，生长速率分别降低56.14％，52.63％和47.37％。所有这些结果突显了TD01是有前景的下一代工业用廉价生物酸盐化学生物制造的工业动力。