Identification of alternatives to antibiotics in livestock and poultry is necessary. Fueled by consumer preferences, phytogenic feed additives are increasingly used in the food system; however, their mode of action is not well defined. Here, we used broiler chickens, in which appetite and feeding behavior regulation are controlled by complex mechanisms, to determine the effect of the phytogenic feed additive “comfort” (PFA-C) as well as its underlying molecular mechanisms on growth performance in heat-stressed broiler chickens. Heat stress significantly increased birds' core body temperature, water intake, and the hypothalamic expression of heat shock protein (HSP) 70, whereas it decreased feed intake, BW, and woody breast incidence. Phytogenic feed additive “comfort” supplementation downregulated the hypothalamic expression of HSP70, reduced core body temperature, increased feed and water intake, and improved BW in HS broilers. At molecular levels, the effect of PFA-C on growth performance seemed to be mediated by modulation of hypothalamic expression of melanocortin receptor 2, arginine vasopressin, aquaporin 2, and sodium and potassium-transporting ATPase subunit beta 1 polypeptides. In summary, PFA-C supplementation ameliorates heat stress productivity losses via a potential cytoprotective effect, reduction of hypothalamic intracellular stress, and modulation of hypothalamic feeding- and drinking-related polypeptide expression.

确定畜禽中抗生素的替代品是必要的。在消费者偏好的推动下，食品系统中越来越多地使用植物性饲料添加剂；然而，他们的行动方式并没有明确界定。在这里，我们使用肉鸡，其中食欲和摄食行为的调节受复杂机制控制，以确定植物性饲料添加剂“舒适度”（PFA-C）及其潜在分子机制对高温下生长性能的影响。压力过大的肉鸡。热应激显着增加了鸟类的核心体温、水摄入量和热休克蛋白 (HSP) 70 的下丘脑表达，而它降低了采食量、体重和木质乳房的发生率。植物源性饲料添加剂“舒适”补充剂下调 HSP70 的下丘脑表达，降低核心体温，增加采食量和饮水量，提高 HS 肉鸡的体重。在分子水平上，PFA-C 对生长性能的影响似乎是通过调节黑皮质素受体 2、精氨酸加压素、水通道蛋白 2 和钠和钾转运 ATP 酶亚基β1 多肽的下丘脑表达来介导的。总之，PFA-C 补充剂通过潜在的细胞保护作用、减少下丘脑细胞内应激和调节下丘脑进食和饮水相关的多肽表达来改善热应激生产力损失。精氨酸加压素、水通道蛋白 2 和钠和钾转运 ATP 酶亚基β 1 多肽。总之，PFA-C 补充剂通过潜在的细胞保护作用、减少下丘脑细胞内应激和调节下丘脑进食和饮水相关的多肽表达来改善热应激生产力损失。精氨酸加压素、水通道蛋白 2 和钠和钾转运 ATP 酶亚基β 1 多肽。总之，PFA-C 补充剂通过潜在的细胞保护作用、减少下丘脑细胞内应激和调节下丘脑进食和饮水相关的多肽表达来改善热应激生产力损失。