The seawater temperature rise can promote the growth of potentially pathogenic Vibrio species. In the North Sea, V. parahaemolyticus strains have been isolated and characterized. These strains contain prophages that may contribute to the emergence of pathogenic strains in the marine environment. Here, we present the genome structure and possible biological functions of the inducible phage vB_VpaI_VP-3218, a novel filamentous phage carried by the V. parahaemolyticus strain VN-3218. Prophages of the strain VN-3218 were induced with mitomycin C and the DNA from the phage induction was sequenced. Two incomplete prophages were identified, only one complete phage genome with length of 11,082 bp was characterized. The phage vB_VpaI_VP-3218 belongs to the Inoviridae family and shows close homology to the Saetivirus genus. This phage can integrate into the chromosomal host genome and carries host-related regions absent in similar phage genomes, suggesting that this phage might integrate in other Vibrio host genomes from the environment. Furthermore, this phage might have a role in pathogenicity due to potential zonula occludens toxin genes. Based on its genomic similarity, the genome of vB_VpaI_VP-3218 phage probably integrates into the lysogen's chromosome and replicates as episome. This study complements prophage induction and bioinformatic studies applied to non-model species of potentially pathogenic Vibrio species. The characterization of this phage provides new insights with respect to the presence of filamentous phages in environmental V. parahaemolyticus strains, which might have a role in the emergence of new pathogenic strains in the North Sea.

海水温度升高会促进潜在致病弧菌的生长。在北海，V。副溶血性菌株已被分离和鉴定。这些菌株含有可能导致海洋环境中出现致病菌株的噬菌体。在这里，我们展示了诱导型噬菌体 vB_VpaI_VP-3218 的基因组结构和可能的生物学功能，这是一种由 V 携带的新型丝状噬菌体。副溶血性杆菌菌株 VN-3218。用丝裂霉素 C 诱导菌株 VN-3218 的前噬菌体，并对来自噬菌体诱导的 DNA 进行测序。鉴定出两个不完整的噬菌体，仅表征了一个长度为 11,082 bp 的完整噬菌体基因组。噬菌体 vB_VpaI_VP-3218 属于Inoviridae家族，与Saetivirus属具有密切的同源性。这种噬菌体可以整合到染色体宿主基因组中，并携带类似噬菌体基因组中不存在的宿主相关区域，表明这种噬菌体可能整合到其他弧菌中宿主环境中的基因组。此外，由于潜在的闭合带毒素基因，这种噬菌体可能在致病性中起作用。基于其基因组相似性，vB_VpaI_VP-3218 噬菌体的基因组可能整合到溶源菌的染色体中并作为附加体复制。该研究补充了应用于潜在致病性弧菌物种的非模型物种的噬菌体诱导和生物信息学研究。这种噬菌体的表征为环境V中丝状噬菌体的存在提供了新的见解。parahaemolyticus菌株，这可能在北海新致病菌株的出现中发挥作用。