As the sister group to bilaterian animals, cnidarians are very valuable models for comparative transcriptomics, shedding light on the evolution and genetic regulation of developmental trajectories, morphogenesis, body patterning and regeneration. To gain deeper insights into the evolution of developmental processes, wider sampling of biological diversity should be performed. In this study, we report the sequencing, assembly and analysis of the transcriptome for the first sertulariid hydrozoan, Dynamena pumila. We sequenced a mixed cDNA library from several developmental stages using Illumina HiSeq 4000. These sequences were assembled into 76,196 contigs with an N50 of 1890 bp. The pool of assembled sequences represents a wide variety of genes expressed during Dynamena development. Functional annotation with Gene Ontology and KEGG pathway analysis has been performed. With this, we identified Dynamena transcripts corresponding to the genes involved in major developmental signalling pathways, including Wnt and TGF-β. The data obtained contribute to the further development of evolutionary and functional transcriptomics and provide the reference transcriptome for future studies.

作为双侧动物的姊妹群体，刺胞动物是比较转录组学的非常有价值的模型，为发展轨迹的演变和遗传调控，形态发生，身体构图和再生提供了亮点。为了更深入地了解发展过程的演变，应该对生物多样性进行更广泛的采样。在这项研究中，我们报告了第一个sertulariid的水生动物Dynamena pumila的转录组的测序，组装和分析。我们使用Illumina HiSeq 4000从多个开发阶段对混合的cDNA文库进行了测序。这些序列被组装成76196个重叠群，其N50为1890 bp。组装的序列池代表Dynamena期间表达的多种基因发展。已经执行了带有基因本体论和KEGG途径分析的功能注释。由此，我们确定了Dynamena转录本，该转录本对应于主要发育信号通路中涉及的基因，包括Wnt和TGF-β。获得的数据有助于进化和功能转录组学的进一步发展，并为将来的研究提供参考转录组。