The Acinetobacter baumanni J1 isolated from surface water of the Eastern Pacific Ocean, demonstrated significant algicidal activity on the algae Alexandrium tamarense. Interestingly, this strain showed the ability to produce an acyl-homoserine lactone (AHL) quorum sensing molecule. To better understand its AHL producing mechanism and its ecological functions, the genome of A. baumanni strain J1 was completely sequenced. The genome contained a circular chromosome of 3,948,465 bp with an average GC content of 39.9 mol%. A total of 3707 protein coding genes, 41 tRNA genes and 16 rRNA genes were obtained. In silico genome annotation identified a LuxI putative gene located on contig 4. Subsequent thin-layer chromatography analysis indicated that C8-AHL could be produced by A. baumanni J1, which confirmed the authenticity of the LuxI gene. Taken together, this work describes an algicidal bacterium that is capable of producing an AHL molecule, which may represent a valuable tool for developing microbial methods to control harmful algae.

从东太平洋表层水分离的鲍曼不动杆菌J1对藻亚历山大藻tamarense表现出显着的杀藻活性。有趣的是，该菌株显示出产生酰基高丝氨酸内酯（AHL）群体感应分子的能力。为了更好地了解其AHL产生机理及其生态功能，对鲍曼不动杆菌菌株J1的基因组进行了完整测序。该基因组包含3,948,465 bp的圆形染色体，平均GC含量为39.9 mol％。共获得3707个蛋白质编码基因，41个tRNA基因和16个rRNA基因。在计算机基因组注释中确定了LuxI推定的基因位于重叠群4上。随后的薄层色谱分析表明，鲍曼不动杆菌J1可以产生C8-AHL ，这证实了LuxI基因的真实性。综上所述，这项工作描述了一种能够产生AHL分子的杀藻细菌，这可能是开发控制有害藻类的微生物方法的有价值的工具。