Macrophages are one of the important cell types in the innate immune system that are present in various anatomical regions of the body and promote early control of pathogens. The relative proportion of macrophages in various lymphoid and non-lymphoid regions is small, and as such it is tedious to purify these cells to homogeneity. Culture of bone marrow precursors with macrophage colony-stimulating factor (M-CSF) results in their differentiation to macrophages, however this procedure results in low numbers of differentiated macrophages. Herein we reveal a new approach of generating increased numbers of differentiated macrophages from bone marrow precursors. We show that M-CSF delivered in a plate-bound form results in the differentiation of significantly more macrophages in comparison to soluble M-CSF. Furthermore, the macrophages differentiated with plate-bound M-CSF display increased metabolic activity and cell death following infection with pathogens.

巨噬细胞是先天免疫系统中重要的细胞类型之一，它存在于人体的各个解剖区域中，可促进病原体的早期控制。在各种淋巴和非淋巴区域中巨噬细胞的相对比例很小，因此将这些细胞纯化至同质是乏味的。带有巨噬细胞集落刺激因子（M-CSF）的骨髓前体的培养导致它们分化为巨噬细胞，但是这种方法导致分化巨噬细胞的数量较少。本文中，我们揭示了一种从骨髓前体中产生分化巨噬细胞数量增加的新方法。我们表明，以可溶性的M-CSF板结合形式交付的M-CSF导致明显更多的巨噬细胞分化。此外，