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Differential induction and suppression of the potato innate immune system in response to Alfalfa mosaic virus infection
Physiological and Molecular Plant Pathology ( IF 2.8 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.pmpp.2020.101485
Ahmed Abdelkhalek , Abdulaziz Abdulrahman Al-Askar , Elsayed Hafez

Abstract Alfalfa mosaic virus (AMV) causes significant losses in the global agricultural production of crops such as potato (Solanum tuberosum L.). Transcriptional levels of 10 innate defense genes, five related to the polyphenol biosynthetic pathway (PAL, HQT, HCT, CHS, and CHI) and five pathogenesis-related proteins (PR-1, PR-2, PR-3, PR-5 and PR-7) were investigated at different days post-inoculation (dpi). The results revealed that fluctuations in the expression of these examined defense genes between induction and suppression, especially in the first 10 dpi, are common. Interestingly, greater up-regulation of most of the tested genes at 4 dpi was associated with greater down-regulation of PR-2 and a reduction in viral coat protein (CP) accumulation. Conversely, greater activation and accumulation of the virus at 10 dpi suppressed the expressions of all genes except PR-1 (salicylic marker gene), and CHI genes showed 1.189- and 1.028-fold changes respectively. At 30 dpi, the greatest level of AMV-CP accumulation (42.27-fold) was associated with severe symptoms that started appearing at 18 dpi. Consequently, at 30 dpi, none of these genes had an effect on the presence and multiplication of the virus inside the plant cell. This study provides the first valuable information of the molecular transcriptional levels of these potato genes under AMV infection.

中文翻译:

马铃薯先天免疫系统对苜蓿花叶病毒感染的差异诱导和抑制

摘要 苜蓿花叶病毒 (AMV) 给全球农作物如马铃薯 (Solanum tuberosum L.) 的农业生产造成重大损失。10 种先天防御基因的转录水平,其中 5 种与多酚生物合成途径相关(PAL、HQT、HCT、CHS 和 CHI)和五种发病机制相关蛋白(PR-1、PR-2、PR-3、PR-5 和PR-7) 在接种后的不同天数 (dpi) 进行了研究。结果表明,这些检查的防御基因在诱导和抑制之间的表达波动,特别是在前 10 dpi,是常见的。有趣的是,在 4 dpi 时大多数测试基因的更大上调与 PR-2 的更大下调和病毒外壳蛋白 (CP) 积累的减少有关。反过来,病毒在 10 dpi 的更大激活和积累抑制了除 PR-1(水杨酸标记基因)之外的所有基因的表达,CHI 基因分别显示 1.189 和 1.028 倍的变化。在 30 dpi 时,AMV-CP 积累的最高水平(42.27 倍)与在 18 dpi 时开始出现的严重症状相关。因此,在 30 dpi 时,这些基因中没有一个对植物细胞内病毒的存在和繁殖产生影响。这项研究提供了这些马铃薯基因在 AMV 感染下的分子转录水平的第一个有价值的信息。因此,在 30 dpi 时,这些基因中没有一个对植物细胞内病毒的存在和繁殖产生影响。这项研究提供了这些马铃薯基因在 AMV 感染下的分子转录水平的第一个有价值的信息。因此,在 30 dpi 时,这些基因中没有一个对植物细胞内病毒的存在和繁殖产生影响。这项研究提供了这些马铃薯基因在 AMV 感染下的分子转录水平的第一个有价值的信息。
更新日期:2020-04-01
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