Abstract In the genome sequence of the fungal pathogen Sclerotinia sclerotiorum, a phosphopantetheinyl transferase gene homolog Ss-Ppt1 (Accession XM_ 001596461.1) with 1 SCOP and 1 ACPS module was identified. And the transcription of Ss-Ppt1 was increased significantly at the sclerotial maturation stage. Silenced mutant strains of Ss-Ppt1 were generated by RNA interference method. The mutant strains were screened and characterized. Compared to the wild-type strain, the hyphal growth of the mutant strains was significantly decreased and the branching pattern was altered. Aberrant sclerotia were produced by the mutant strains with larger volume but reduced number. Moreover, the virulence of the mutant strains was attenuated, and the mutant strains failed to generate necrotic disease symptoms as the wide-type strain. These results indicated that Ss-Ppt1 protein plays an important role in hyphal growth, sclerotial development and pathogenicity of S. sclerotiorum.

中文翻译：

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磷酸泛酰巯基乙胺转移酶基因 Ss-Ppt1 的沉默影响核盘菌的多种发育途径和致病性

摘要 在真菌病原菌核盘菌的基因组序列中，鉴定出一个磷酸泛酰巯基乙胺转移酶基因同源物Ss-Ppt1（登录号XM_001596461.1），具有1个SCOP和1个ACPS模块。Ss-Ppt1的转录在菌核成熟阶段显着增加。Ss-Ppt1 的沉默突变株是通过 RNA 干扰方法产生的。筛选和表征突变菌株。与野生型菌株相比，突变菌株的菌丝生长显着降低，分支模式发生改变。体积较大但数量减少的突变菌株产生了异常菌核。此外，突变株的毒力减弱，并且突变株未能产生作为宽型菌株的坏死病症状。