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Identification, expression pattern, and immune response of Tim-1 and Tim-4 in embryos and adult medaka (Oryzias latipes).
Journal of Experimental Zoology-B: Molecular and Developmental Evolution ( IF 1.8 ) Pub Date : 2020-03-09 , DOI: 10.1002/jez.b.22939 Hao Shen 1 , Emile Nibona 1 , Gongyu Xu 1 , Md Abdullah Al Hafiz 1 , Xiaomei Ke 1 , Xiaoting Liang 1 , Qiting Yao 1 , Xueping Zhong 1 , Qingchun Zhou 1 , Haobin Zhao 1
Journal of Experimental Zoology-B: Molecular and Developmental Evolution ( IF 1.8 ) Pub Date : 2020-03-09 , DOI: 10.1002/jez.b.22939 Hao Shen 1 , Emile Nibona 1 , Gongyu Xu 1 , Md Abdullah Al Hafiz 1 , Xiaomei Ke 1 , Xiaoting Liang 1 , Qiting Yao 1 , Xueping Zhong 1 , Qingchun Zhou 1 , Haobin Zhao 1
Affiliation
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T-cell immunoglobulin (Ig) and mucin domain-containing 1 (Tim-1) and Tim-4 are two members of the Tim family. In mammals, Tim-1 and Tim-4 are proteins mainly expressed in immune cells and are associated with immune response. In the present study, medaka Oryzias latipes' Tim-1 (OlTim-1) and OlTim-4 were identified and characterized using bioinformatics analyses. With the use of reverse-transcription polymerase chain reaction, the expression profiles of OlTim-1 and OlTim-4 were examined in embryos and adult fish and in immune tissues following the intraperitoneal injection of stimulants. The results revealed that OlTim-1 possesses a cytoplasmic region, a transmembrane region, a mucin domain, and an Ig-like domain, while OlTim-4 is composed of two Ig-like domains and a mucin domain, but without the transmembrane region and cytoplasmic region. OlTim-1 and OlTim-4 expressions are detectable from the gastrula stage on, indicating that they are zygotic genes. Furthermore, OlTim-1 and OlTim-4 are expressed ubiquitously in the adult. Administration of immune stimulants, namely lipopolysaccharides and polyinosinic:polycytidylic acid, significantly increased the expression levels of OlTim-1 and OlTim-4 in the liver and intestine within 1 day and in the head, kidney, and spleen within 3 to 4 days postinjection. These results suggest that OlTim-1 and OlTim-4 are possibly involved in both innate and adaptive immunities.
中文翻译：
Tim-1和Tim-4在胚胎和成年青aka（Oryzias latipes）中的鉴定，表达模式和免疫应答。
T细胞免疫球蛋白（Ig）和含粘蛋白结构域的1（Tim-1）和Tim-4是Tim家族的两个成员。在哺乳动物中，Tim-1和Tim-4是主要在免疫细胞中表达的蛋白质，与免疫反应有关。在本研究中，使用生物信息学分析鉴定和鉴定了（Myaka Oryzias latipes）的Tim-1（OlTim-1）和OlTim-4。使用逆转录聚合酶链反应，腹膜内注射刺激剂后，在胚胎和成鱼中以及免疫组织中检查了OlTim-1和OlTim-4的表达情况。结果显示，OlTim-1具有胞质区，跨膜区，粘蛋白结构域和Ig样结构域，而OlTim-4由两个Ig样结构域和粘蛋白结构域组成，但没有跨膜区和细胞质区域。从下腹阶段开始可检测到OlTim-1和OlTim-4表达，表明它们是合子基因。此外，在成年人中普遍表达OlTim-1和OlTim-4。注射免疫刺激剂（即脂多糖和多肌苷酸：多聚胞苷酸）后，注射后1天之内，肝脏和肠中以及头部，肾脏和脾脏中的OlTim-1和OlTim-4的表达水平显着增加。这些结果表明，OlTim-1和OlTim-4可能参与先天和适应性免疫。聚胞苷酸在注射后1天之内显着增加肝脏和肠道中的OlTim-1和OlTim-4的表达水平，在注射后3至4天内在头部，肾脏和脾脏中的表达水平明显升高。这些结果表明，OlTim-1和OlTim-4可能参与先天性和适应性免疫。聚胞苷酸在注射后1天之内显着增加肝脏和肠道中的OlTim-1和OlTim-4的表达水平，在注射后3至4天内在头部，肾脏和脾脏中的表达水平明显升高。这些结果表明，OlTim-1和OlTim-4可能参与先天和适应性免疫。
更新日期：2020-03-09
中文翻译：
Tim-1和Tim-4在胚胎和成年青aka（Oryzias latipes）中的鉴定，表达模式和免疫应答。
T细胞免疫球蛋白（Ig）和含粘蛋白结构域的1（Tim-1）和Tim-4是Tim家族的两个成员。在哺乳动物中，Tim-1和Tim-4是主要在免疫细胞中表达的蛋白质，与免疫反应有关。在本研究中，使用生物信息学分析鉴定和鉴定了（Myaka Oryzias latipes）的Tim-1（OlTim-1）和OlTim-4。使用逆转录聚合酶链反应，腹膜内注射刺激剂后，在胚胎和成鱼中以及免疫组织中检查了OlTim-1和OlTim-4的表达情况。结果显示，OlTim-1具有胞质区，跨膜区，粘蛋白结构域和Ig样结构域，而OlTim-4由两个Ig样结构域和粘蛋白结构域组成，但没有跨膜区和细胞质区域。从下腹阶段开始可检测到OlTim-1和OlTim-4表达，表明它们是合子基因。此外，在成年人中普遍表达OlTim-1和OlTim-4。注射免疫刺激剂（即脂多糖和多肌苷酸：多聚胞苷酸）后，注射后1天之内，肝脏和肠中以及头部，肾脏和脾脏中的OlTim-1和OlTim-4的表达水平显着增加。这些结果表明，OlTim-1和OlTim-4可能参与先天和适应性免疫。聚胞苷酸在注射后1天之内显着增加肝脏和肠道中的OlTim-1和OlTim-4的表达水平，在注射后3至4天内在头部，肾脏和脾脏中的表达水平明显升高。这些结果表明，OlTim-1和OlTim-4可能参与先天性和适应性免疫。聚胞苷酸在注射后1天之内显着增加肝脏和肠道中的OlTim-1和OlTim-4的表达水平，在注射后3至4天内在头部，肾脏和脾脏中的表达水平明显升高。这些结果表明，OlTim-1和OlTim-4可能参与先天和适应性免疫。
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