In this study, activated carbon/diatomite‐based magnetic nancomposites (denoted as AC/DBMNs) were synthesized and applied as an adsorbent for magnetic solid‐phase extraction of S‐ phenylmercapturic acid (S‐ PMA) from human urine prior to high‐performance liquid chromatography. The surface morphologies and structures of AC/DBMNs were characterized by Fourier transform infrared spectroscopy, transmission and scanning electron microscopy, X‐ray diffraction, Brunauer–Emmett–Teller surface area, vibrating sample magnetometer and ζ ‐potential measurements. The experimental parameters including sample volume, sample pH, adsorbent amount, extraction time, elution solvent and desorption time were investigated in detail. Under the optimum conditions, the method exhibited good linearity (r  > 0.9993) within the concentration ranges of 0.03–1.0 mg/L. Moreover, the limits of detection and quantification were 0.01 and 0.03 mg/L, respectively. The enrichment factor was 5, and good recoveries (88.9–97.3%) with relative standard deviations in the range of 5.6–6.8% (n  = 6) for inter‐day and 6.3–8.1% (n  = 6) for intra‐day were achieved. The developed method was successfully applied to the analysis of S‐ PMA in urine samples. In addition, this accurate and sensitive method has great potential to be applied in the early screening and clinical diagnosis of the workers exposed to benzene.

中文翻译：

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活性炭/硅藻土基磁性纳米复合材料，用于从人尿液中磁性固相萃取S-苯基巯基酸。

在这项研究中，合成了基于活性炭/硅藻土的磁性纳米复合材料（表示为AC / DBMNs），并用作吸附剂，用于从人尿中进行磁性固相萃取S-苯基巯基酸（S- PMA），然后再进行高性能处理。液相色谱。AC / DBMNs的表面形态和结构通过傅里叶变换红外光谱，透射和扫描电子显微镜，X射线衍射，Brunauer-Emmett-Teller表面积，振动样品磁力计和ζ进行表征。电位测量。详细研究了样品体积，样品pH，吸附量，萃取时间，洗脱溶剂和解吸时间等实验参数。在最佳条件下，该方法在 0.03–1.0 mg / L的浓度范围内表现出良好的线性（r > 0.9993）。此外，检测限和定量限分别为0.01和0.03 mg / L。富集因子为5，和良好的回收率（88.9-97.3％）具有在5.6-6.8％（范围相对标准偏差Ñ  = 6），用于日间和6.3-8.1％（Ñ  = 6）为日内实现了。所开发的方法已成功应用于S‐尿液样品中的PMA。另外，这种准确而灵敏的方法在接触苯工人的早期筛查和临床诊断中具有很大的潜力。