An ultra‐high performance liquid chromatography tandem mass spectrometry method was developed for determination of homocysteine (HCY) in human plasma. The HCY was derivatized with 2‐chloro‐1‐methylquinolinium tetrafluoroborate and isolated using solid‐phase extraction. Derivatization, isolation and detection procedures were optimized. Satisfactory linearity was obtained with determination coefficients (r ²) >0.999. The intra‐ and inter‐day precisions were in the interval of 1.2–5.1% and accuracy was within ±7%. Mean recoveries were close to 100%. The limit of detection and the limit of quantification were 0.46 and 1.38 μmol/L, respectively. The method was then applied to investigate the relationship between plasma HCY and whole blood 5‐methyltetrahydrofolate levels in healthy volunteers. The results revealed that the plasma level of HCY was significantly negatively correlated to whole blood 5‐methyltetrahydrofolate in volunteers.

中文翻译：

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用UHPLC-MS / MS方法测定血浆高半胱氨酸：用于分析健康志愿者血浆高半胱氨酸与全血5-甲基四氢叶酸之间的相关性。

建立了一种用于测定人血浆中同型半胱氨酸（HCY）的超高效液相色谱串联质谱法。HCY用2-氯-1-甲基喹啉四氟硼酸酯衍生并使用固相萃取进行分离。优化了衍生化，分离和检测程序。通过确定系数获得了令人满意的线性度（r ²）> 0.999。日内和日间精度在1.2–5.1％之间，精度在±7％之内。平均回收率接近100％。检测限和定量限分别为0.46和1.38μmol/ L。然后将该方法用于研究健康志愿者血浆中HCY与全血5-甲基四氢叶酸水平之间的关系。结果表明，血浆中HCY水平与志愿者全血5-甲基四氢叶酸显着负相关。