We isolated 18 rhizobial strains from root nodules of a leguminous shrub Laburnum anagyroides (common laburnum) grown in Southeast Poland as an exotic plant. With the use of BOX-PCR fingerprinting, the isolates were clustered into 2 main groups and one separate lineage, which was congruent with the ITS-RFLP results. The phylogenetic trees constructed based on 16S rRNA and combined atpD, dnaK, glnA, and recA gene sequence data separated the representative strains into three evolutionary lineages within the Bradyrhizobium jicamae supergroup, with Bradyrhizobium algeriense and Bradyrhizobium valentinum as the closest relatives. The nodA and nifH gene phylogenies proved that the L. anagyroides symbionts carry a symbiotic gene variant known as Clade IV, representing the symbiovar retamae. Phenotypic characteristics of the isolates and reference strains are also reported. Our study of the rhizobia nodulating L. anagyroides growing in Poland complements earlier few findings on the symbiotic associations of this Genisteae species.

中文翻译：

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缓生根瘤菌与金链花豆（Laburnum anagyroides）有关，后者是波兰种植的一种异国豆类

我们从波兰东南部作为外来植物生长的豆科灌木金链花（普通金链花）的根瘤中分离出18种根瘤菌菌株。使用BOX-PCR指纹图谱，将分离株分为2个主要组和一个独立的谱系，这与ITS-RFLP结果一致。基于16S rRNA并结合atpD，dnaK，glnA和recA基因序列数据构建的系统树将具有代表性的菌株分为奇特氏缓生根瘤菌超群内的三个进化谱系，其中有阿尔及的缓生根瘤菌和终生的缓生根瘤菌。作为最亲戚 所述的nodA和nifH基因的系统发育证明了L. anagyroides共生体携带称为进化枝IV共生基因变异，代表symbiovar retamae。还报道了分离株和参考菌株的表型特征。我们对波兰生长的根瘤菌结节线虫L. zogyroides的研究补充了关于该金雀科物种共生关系的早期发现。